中华蜜蜂6日龄幼虫肠道响应球囊菌胁迫的差异表达基因分析  

Analysis of the differentially expressed genes in the 6-day-old larval gut of Apis cerana cerana under the stress of Ascosphaera apis

作　　者：郭睿[1] 张璐[1] 徐细建[1] 史秀丽 熊翠玲[1] 郑燕珍[1] 付中民[1] 黄枳腱 王鸿权 侯志贤[1] 陈大福[1] 

机构地区：[1]福建农林大学蜂学学院,福州350002 [2]新疆维吾尔自治区蜂业技术管理总站,乌鲁木齐930001

出　　处：《环境昆虫学报》2017年第3期539-547,共9页Journal of Environmental Entomology

基　　金：现代农业产业技术体系建设专项资金(CARS-45-KXJ7);福建农林大学科技发展资金(KF2015123)

摘　　要：蜜蜂球囊菌特异性侵染蜜蜂幼虫而导致白垩病,严重危害养蜂生产。本研究利用RNA-seq技术对健康及球囊菌胁迫的中蜂幼虫肠道进行深度测序,进而对宿主的差异表达基因进行深入分析。本研究中,幼虫肠道样品的RNA-seq共得到191167730条原始读段(raw reads),经过滤得到186284296条有效读段(clean reads),差异表达基因(DEG)分析结果显示上调与下调基因的数量分别为4513和385个。Gene ontology(GO)富集分析结果显示,上调基因富集在45个GO条目(term),富集基因数最多的是细胞进程、代谢进程及催化活性,下调基因富集在32个GO term,富集基因数最多的是代谢进程、单组织进程及催化活性,KEGG代谢通路(pathway)富集分析结果显示上调基因富集在193个pathway,其中富集基因数最多的是核糖体、氨基酸的合成、碳代谢。下调基因富集在59个pathway,其中富集基因数最多的是甘氨酸、碳代谢以及二羧酸代谢。深入分析发现宿主的细胞免疫被显著激活,体液免疫中的Toll-like与Jak-STAT信号通路也被球囊菌所激活。研究结果为揭示中蜂幼虫在球囊菌入侵后期的胁迫应答机制提供了重要的信息,也为解析中蜂幼虫的球囊菌抗性机制奠定了基础。Ascosphaera apis specially infect honeybee larvae and leads to chalkbrood,which causes a huge loss for apiculture. Here,untreated and A. apis-treated Apis cerana cerana larval guts were sequenced using RNA-seq technology, followed by analysis of differentially expressed genes（ DEGs）. In this study,Illumina sequencing of larval guts generated a total of 191,1677,30 raw reads,and after filtration,186,284,296 clean reads were obtained. DEGs analysis showed that 4513 genes were up-regulated and 385 were down-regulated. GO enrichment analysis displayed that the up-regulated genes were enriched in 45 terms,among them the mostly enriched ones were cell process,metabolic process and catalyitic activity;the down-regulated genes were enriched in 32 terms,and the mostly enriched ones were metabolic process,single organism process as well as catalyitic activity. Furthermore,KEGG enrichment analysis suggestedthat the up-regualted genes were engaged in 193 pathways,the mostly enriched one was ribosome,followed by biosynthesis of acino acids and carbon metabolism; the down-regualted genes were involved in 59 pathways,and the mostly enriched ones were glycine, carbon metabolism as well as glyoxylate and dicarboxylate metabolism. Further analysis showed that the host＇s cellular immune was activated and the humoral immunity such as Toll-like and Jak-STAT signaling pathways were significantly induced to activation. Findings in the present study can not only provide key information for uncovering the mechanism regulating the A. c. cerana larvae＇s responses to A. apis during the late stage of stress,but also lay a foundation for clarifying the A. apis-resistance mechanism of A. c. cerana larvae.

关 键 词：中华蜜蜂 幼虫肠道 球囊菌 差异表达基因 免疫防御 

分 类 号：S891[农业科学—特种经济动物饲养]