冬虫夏草线状菌体制剂对蝠蛾幼虫感染的研究  

The infection research on the Ophiocordyceps sinensis filamentous mycelium to Hepialus moths larvae

作　　者：贺宗毅[1,2,3,4] 张德利[1,2,3,4] 李黎[1,4] 邢康康 陈仕江[1,2,3,4] 

机构地区：[1]重庆市中药研究院,重庆400065 [2]重庆市中药资源学重点实验室,重庆400065 [3]中国中医科学院,中药资源中心重庆分中心,重庆400065 [4]甘孜州康定贡嘎中华虫草产业有限责任公司,四川甘孜626000

出　　处：《环境昆虫学报》2017年第3期673-678,共6页Journal of Environmental Entomology

基　　金：重庆市科技平台项目(cstc2014ptyjd10001);重庆市科技惠民计划项目(2013gs500102-D2014-3);重庆市社会民生科技创新专项(cstc2015shmszx120032);重庆市科技攻关计划项目(cstc2013yykf B110002)

摘　　要：为明确冬虫夏草线状菌体制剂的侵染力,进行了冬虫夏草线状菌体制剂对蝠蛾幼虫的感染试验。在相同条件下对蝠蛾幼虫进行人工接种,并对感染温度、菌剂用量和虫龄进行了优化。结果表明冬虫夏草线状菌体具有侵染蝠蛾幼虫的能力,优化后的感染条件为:菌体剂量为5 g(即5个标准剂量)、幼虫虫龄为3龄、培养温度为14℃。该结果为蝠蛾幼虫新型接种体的制备与开发提供了依据。In order to clear the infection of the Ophiocordyceps sinensis filamentous mycelium to the Hepialus moths larvae,the infection of O. sinensis filamentous mycelium to Hepialus moths larvae was tested by artificial fed at the same environment and condition. Then,the infected conditions were optimized by orthogonal experiment design. The results showed that the infected conditions were amount of O. sinensis filamentous mycelium 5 g,instar 3th,temperature 14℃,In summery,the O. sinensis filamentous mycelium had the infection to the Hepialus moths larvae. The result had provided theoretical basis to the reforming of O. sinensis filamentous mycelium inoculant preparation.

关 键 词：冬虫夏草菌 线状菌体 蝠蛾幼虫 感染 

分 类 号：Q965[生物学—昆虫学]