急性下呼吸道感染患儿中RSV的分离鉴定及分析  

Isolation,identification and analysis of RSV in children with acute lower respiratory tract infection

作　　者：傅生芳[1] 张蓉芳 朱传凤[1] 安静[1] 余黎[1] 

机构地区：[1]兰州生物制品研究所有限责任公司第二研究室甘肃省疫苗工程技术研究中心,甘肃兰州730046 [2]甘肃省妇幼保健医院儿童哮喘中心,甘肃兰州730050

出　　处：《微生物学免疫学进展》2017年第3期14-18,共5页Progress In Microbiology and Immunology

基　　金：国家自然科学基金项目(81260001)

摘　　要：目的对2015年兰州单中心急性下呼吸道感染患儿中呼吸道合胞病毒(respiratory syncytial virus,RSV)进行分离鉴定及分析。方法收集临床急性下呼吸道感染患儿痰分泌物226份,在HEP-2细胞中连续盲传培养3代,收获的培养物用半巢式RT-PCR进行检测,选取强阳性样品进行测序以确定其型别,并对其流行病学特征进行分析。结果 226份样品中RSV阳性样本为76份(阳性率为34%)。RSV阳性患儿中,男女性别比为3∶1;年龄分布<6月龄患儿占45%,6月龄~2岁占35%,>2~5岁的占20%。冬季为发病高峰。76例RSV阳性患儿中,临床诊断依次为毛细支气管炎31例(40.79%)、喘息性支气管炎21例(27.36%)、支气管肺炎20例(26.32%)和支气管哮喘4例(5.26%)。40份强阳性RSV样品经测序确定为B亚型。结论 RSV是引起婴幼儿急性下呼吸道感染的重要病原之一,2015年RSV的流行株主要为B亚型。Objective To isolate, identify and analyze respiratory syncytial virus （ RSV } infection in children with acute lower respiratory tract infection in Lanzhou single center, in 2015. Methods A total of 226 sputum secretion samples of acute lower respiratory tract infection were collected and blindly seeded in HEP-2 cell line for 3 consecutive passages. The cultures were hal＇vested and detected by semi-nested RT-PCR, respectively. The strong and specific positive samples were sequenced to determine the type in RSV, and the epidemic characteristics were investigated. Results A total of 76 sampies were RSV positive among 226 samples, with a positive rate 34%. The case ratio for male to female was 3 ： 1 in chil- dren with RSV positive. Among these children, infants and younger children under 6 months accounted for 45% , 7-24 months accounted for 35% , and 〉2-5 years accounted for 20%. The RSV infection mainly occured in the winter. There were 76 cases with RSV infection by clinical diagnosed, 31 （40.79%） , 21 （27.36%） , and 20（26.32% ） cases suffered from bronchiolitis, asthmatic bronchitis, and bronchial pneumonia, respectively, the other 4 cases were bronchial asthma （ 5.26% ）. There were 40 strong positive samples determined as subtype B by sequencing. Conclusion RSV is one of the most important pathogens causing acute lower respiratory tract infection in infants and younger children. The predominate epidemic strains of RSV attributed to B subtype in 2015 by single center analysis in Lanzhou.

关 键 词：急性下呼吸道感染 呼吸道合胞病毒 分离 鉴定 分析 

分 类 号：R725.6[医药卫生—儿科]