基于表面重建螺旋型铂铱电极的葡萄糖生物传感器  

Glucose Biosensor Based on Rebuilding the Surface of the Spiral-type Pt-Ir Electrode

作　　者：潘帅[1] 李崭虹 陈杨[1] 赵雪伶[1] 陈诚[1] 朱志刚[1] 

机构地区：[1]上海第二工业大学工学部环境与材料工程学院,上海201209

出　　处：《高等学校化学学报》2017年第7期1163-1170,共8页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:61471233;21504051);上海高校特聘教授(东方学者)计划项目;上海市教委曙光计划项目(批准号:14SG52)资助~~

摘　　要：通过对螺旋型铂铱电极表面进行化学腐蚀和电化学沉积铂纳米粒子实现电极表面的重建和优化,研究了螺旋型铂铱电极在不同腐蚀时间和电沉积时间下的形貌及对过氧化氢(H2O2)的催化活性.对表面重建的工作电极涂覆氧化酶和半透膜,制备出了铂纳米粒子/葡萄糖氧化酶/环氧聚氨酯酶电极,并将其用作葡萄糖传感器的工作电极.传感器计时电流检测结果表明,表面重建后的酶电极传感器对葡萄糖的检测范围扩大为2~45 mmol/L,优于裸铂铱酶电极传感器,电流响应值和灵敏度得到明显提升,同时传感器还具有良好的稳定性和选择性.The surface of the spiral-type platinum-iridium electrode was rebuilt and optimized by chemical etching and electrochemical deposition of platinum nanoparticles（PtNPs）. The effects of different etching time and electrodeposition time on the morphology and the catalytic activity of the electrode toward hydrogen peroxide were studied. After coating glucose oxidase（GOD） and epoxy-polyurethanes（Epoxy-PU） to surface-rebuih electrode, the PtNPs/GOD/Epoxy-PU enzymatic biosensor was successfully fabricated. The chronoamperometric test indicates that the detection range of PtNPs/GOD/Epoxy-PU biosensor is expanded to 2--45 mmol/L, which is much wider than that of bare electrode based biosensor. PtNPs/GOD/Epoxy-PU biosensor has high response current and sensitivity, good stability and selectivity as well.

关 键 词：葡萄糖传感器 螺旋型铂铱电极 化学腐蚀 电化学沉积 检测范围 

分 类 号：O657.1[理学—分析化学]