rRT-PCR与微量中和实验法用于脊灰病毒血清定型的比较  

Comparison of rRT-PCR and Micro-neutralization Assay for Serotyping of Polivirus

作　　者：陈玫[1] 张俊棉[1] 赵娜[1] 郭玉[1] 张振国[1] 

机构地区：[1]河北省疾病预防控制中心,石家庄050021

出　　处：《预防医学情报杂志》2017年第8期732-735,共4页Journal of Preventive Medicine Information

基　　金：河北省科技支撑计划项目(14277726D)

摘　　要：目的对实时荧光定量逆转录-聚合酶链反应(rRT-PCR)法和微量中和实验2种脊灰病毒的血清型鉴定方法进行评价。方法按世界卫生组织(WHO)《脊髓灰质炎实验室手册》第4版进行病毒分离,用rRT-PCR法和微量中和实验方法对分离到的L20B阳性分离物进行PV的血清型鉴定,用rRT-PCR法进行型内鉴定。结果 rRT-PCR法与微量中和实验对L20B阳性分离物进行脊灰病毒的血清型定型,2种方法检测脊灰病毒阳性率差异无统计学意义,检测NPEV阳性率差异也无统计学意义;rRT-PCR方法检测脊灰病毒的灵敏度为100%,特异度为100%;检测NPEV的灵敏度为63.16%,特异度为100%。结论作为WHO推荐使用的新的型内鉴定方法,rRT-PCR法对于脊灰病毒的血清型定型与常规微量中和实验一致率为100%,该方法方便快捷,可以缩短检测时限;对于NPEV的检测特异度较高,但灵敏度低于微量中和实验。Objective To evaluate real -time reverse transcription -polymerase chain reaction （rRT- PCR） method and micro -neutralization assay for serotyping of polivirus. Methods Isolation of virus was performed according to the World Health Organization （WHO） Poliomyelitis Laboratory Manual （ the 4th edition）, PV se- rotyping was performed for L20B positive isolates by rRT - PCR and micro - neutralization assay, and intratypic differentiation by rRT - PCR. Results No statistically significant difference was found between polivirus positive rates or NPEV positive rates obtained by the two methods, rRT -PCR was 100% sensitive and 100% specific to polivirus; and 63.16% sensitive and 100% specific to NPEV. Conclusion As a new method for intratypic differentiation recommended by WHO, rRT- PCR yields the same results as routine micro -neutralization method for serotyping of polivirus. The method is simple and convenient and can shorten the test duration; it is highly specific to NPEV but not as sensitive as micro -neutralization method.

关 键 词：急性弛缓性麻痹 脊髓灰质炎病毒 实时荧光定量逆转录-聚合酶链反应 微量中和实验 

分 类 号：R512.4[医药卫生—内科学]