基于肿瘤基因作用的妊娠妇女调节性免疫细胞与巨细胞病毒的相关性研究  

Correlation Between the Regulatory Immune Cells and the Cytomegalovirus in Pregnant Women Based on Tumor Gene Function

作　　者：凌波[1] 王玉珏[1] 何霞[2] 

机构地区：[1]四川省人民医院妇产科,成都610031 [2]四川省人民医院药学部,成都610031

出　　处：《病毒学报》2017年第4期530-534,共5页Chinese Journal of Virology

基　　金：国家自然科学基金(项目号:81301746);题目:新基因C16orf72在肿瘤发生中的作用及分子机制~~

摘　　要：研究基于肿瘤基因作用的妊娠妇女调节性免疫细胞与巨细胞病毒的相关性。收集2015年5月~2016年9月四川省人民医院就诊的妊娠患者,病例纳入标准:年龄范围在22~40岁;初次妊娠患者;排除前置胎盘、先兆流产和妊娠高血压等异常妊娠患者;排除合并慢阻肺、糖尿病、艾滋病等慢性疾病患者;排除艾滋病和乙肝病毒等感染患者;排除长期吸烟、酗酒患者。梯度离心的方法分离患者外周血的淋巴细胞。CD4、CD25和Foxp3流式抗体标记调节性T细胞(Treg细胞);CD19和TGF^-β流式抗体标记调节性B细胞(Breg细胞);CD4、CXCR5和Foxp3流式抗体标记滤泡调节性T细胞(Tfr细胞)。结果表明,对照组共有30例正常妊娠患者,年龄在22岁至37岁之间;CMV感染组包括30名CMV感染的妊娠患者,年龄在22岁至39岁之间。CMV感染组中CD4^+CD25^+Foxp3^+的Treg细胞、CD19^+TGF^-β^+的Breg细胞和CD4^+CXCR5^+Foxp3^+的Tfr细胞比例均显著的低于对照组,分别下降了31.9%、78.5%和65.1%,结果具有统计学差异(P<0.05)。CMV感染后,妊娠患者体内Treg细胞、Breg细胞和Tfr细胞等调节性免疫细胞的比例均显著下降,可能对胎儿的生长发育造成影响。To study the correlation between regulatory immune cells and the cytomegalovirus（CMV）in pregnant women based on tumor gene function.Pregnant patients were recruited from May 2015 to September 2016 in our hospital.Inclusion criteria were age 22~40years and first pregnancy.Exclusion criteria were placenta previa,potential abortion,pregnancy-induced hypertension,abnormal pregnancy,COPD,diabetes mellitus,AIDS,any chronic disease,infection with the hepatitis-B virus,and long-term consumption of tobacco or alcohol.The gradient-centrifugation method was used to isolate lymphocytes from peripheral blood.Antibodies against CD4,CD25 and Foxp3 were used to label regulatory T cells（Treg cells）;CD19and TGF-βantibody was used to label regulatory B cells（Breg cells）;antibodies against CD4,CXCR5 and Foxp3were used to label follicular regulatory T cells（Tfr cells）.The control group comprised 30 healthy pregnant women aged 22~37years.The CMV infection group comprised 30CMV-infected pregnant women aged 22~39years.Percentage of CD4~＋CD25~＋Foxp3~＋Treg cells,CD19~＋TGF^-β~＋Breg cells and CD4~＋CXCR5~＋Foxp3~＋Tfr cells in the CMV infection group was significantly lower than that in the control group,and decreased by 31.9%,78.5% and 65.1%,respectively,a difference that was significant（P0.05）.After CMV infection,the proportion of Treg cells,Breg cells and Tfr cells decreases significantly：this may have adverse effects on the growth and development of fetuses.

关 键 词：妊娠 巨细胞病毒(CMV) 免疫 调节性免疫细胞 

分 类 号：R714[医药卫生—妇产科学]