单颗粒示踪技术及其在病毒侵染机制研究中的应用  

Single-particle Tracking for Study of the Mechanism of Action Viral Infection

作　　者：梁振普[1] 王彩平[1] 张小霞[1] 张俊庆[1] 刘雅静[1] 李鹏娟[1] 冯文霞[1] 

机构地区：[1]河南农业大学生命科学学院,郑州450002

出　　处：《病毒学报》2017年第4期638-645,共8页Chinese Journal of Virology

基　　金：国家自然科学基金(项目号:31490600);题目:猪繁殖与呼吸综合征病毒的致病机理研究~~

摘　　要：病毒侵染宿主细胞是一个复杂的动态过程,且涉及病毒与细胞多种成分的相互作用,病毒侵染机制研究对研制抗病毒药物以及病毒性疾病的预防和治疗至关重要。单颗粒示踪技术是可视化研究生物动态过程的重要工具,能够研究病毒在活细胞中的侵染行为,现已成为病毒侵染机制研究的有效手段。此文综述了单颗粒示踪技术中病毒标记物、标记方法、活细胞成像及分析进展,以流行性感冒病毒和人免疫缺陷病毒为例阐述了该技术在病毒侵染机制研究中的应用,对该技术的应用前景进行了展望。此文旨在为病毒侵染机制研究介绍新的技术手段、推动病毒学研究。Viral infection（IV） is a complex and dynamic process, involving interactions between viruses and all types of cellular components. Study of virus infection mechanism is very important for the development of antiviral drugs and prevention and treatment of viral diseases. Single-particle tracking （SPT） is an im- portant tool to visually study a dynamic biological process. SPT, can be used to study VI in living cells and has become an effective means to study virus infection mechanism. This review summarizes SPT including the use of virus markers, labeling methods, imaging/ analyses of living clls, as well as, its application to study of virus infection mechanism take influenza and HIV as example. We also reviewed its application foreground, providing new technical means to study the virus infection mechanism and promoting the oro-gress of the research on the virus.

关 键 词：病毒 单颗粒示踪技术 病毒侵染机制 

分 类 号：R37[医药卫生—病原生物学] S852.65[医药卫生—基础医学]