芒果苷对血管内皮细胞中组织因子的影响及其机制  

Effects of mangiferin on tissue factor expression in vascular endothelial cells and the underlying mechanisms

作　　者：王阳阳[1] 于惠玲[1] 陈岩[1] 常颖[1] 魏亚宁[1] 侯英健[1,2] 

机构地区：[1]河北大学附属医院内科,河北保定071000 [2]河北大学医学院,河北保定071000

出　　处：《中国药理学通报》2017年第7期961-965,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81200078);河北省自然科学基金资助项目(No H2013201178);河北大学附属医院院内青年基金资助项目(No 2016Q001);河北大学医学学科基金(No 2012001)

摘　　要：目的探讨芒果苷对组织因子(TF)的调节作用及机制。方法培养人脐静脉内皮细胞(HUVECs),给予多种剂量的芒果苷和(或)氧化低密度脂蛋白(ox LDL)、过氧化物酶体增殖物激活受体γ(PPARγ)抑制剂处理细胞,利用实时定量PCR、Western blot技术以及相关试剂盒检测TF表达及活性的变化。结果 ox LDL上调TF的表达水平并增强其促凝血活性,而这一作用被芒果苷所逆转。而且,芒果苷呈剂量依赖性抑制TF m RNA与蛋白的表达以及促凝活性。芒果苷可增强过氧化物酶体增殖物激活受体γ(PPARγ)的转录活性,PPARγ抑制剂——GW9662则可阻断芒果苷对TF的抑制作用。结论芒果苷通过激活PPARγ而抑制血管内皮细胞中TF的表达及活性。Aim To explore the effects of mangiferin on tissue factor（TF） expression in human umbilical vein endothelial cells（HUVECs） and the underlying mechanisms.Methods HUVECs were isolated and primarily cultured in vitro.After the treatment with mangiferin and oxidized low density lipoprotein（oxLDL）,TF expression was determined in HUVECs with real-time PCR and Western blot.Results ox LDLinduced the mR NA and protein expression and prothrombotic activity of TF in HUVECs.However,the inductive effects of ox LDL were blocked significantly by mangiferin.Furthermore,mangiferin modified TF ex-pression and activity in a dose-dependent manner.Mangiferin was demonstrated to enhance the activity of peroxisome proliferator-activated receptor gamma（PPARγ）.In contrast,GW9662,an antagonist of PPARγ,reversed at least partially the suppressive effects of mangiferin on TF.Conclusion Through activating PPARγ,mangiferin suppresses the expression of TF serving pro-thrombotic functions in endothelial cells.

关 键 词：芒果苷 组织因子 人脐静脉血管内皮细胞 过氧化物酶体增殖物激活受体Γ 血栓 GW9662 

分 类 号：R284.1[医药卫生—中药学] R322.123[医药卫生—中医学]