肝X受体激动剂GW3965对高糖所致心肌细胞凋亡的影响及作用机制  

The effect of liver X receptors agonist GW3965 on myocardial apoptosis induced by high glucose and its mechanism

作　　者：成永霞[1] 张大伟[2] 孙立新[1] 冯玉宽[1] 郭素芬[1] 刘贵波[2] 

机构地区：[1]牡丹江医学院病理学教研室,牡丹江157011 [2]牡丹江医学院解剖教研室,牡丹江157011

出　　处：《陕西医学杂志》2017年第7期819-822,共4页Shaanxi Medical Journal

基　　金：国家青年基金资助项目(81500629)

摘　　要：目的:观察GW3965对高糖诱导的心肌细胞凋亡的影响,并初步探讨其作用机制。方法:取对数期H9c2心肌细胞,随机分为对照组(Control组)、高糖诱导组(高糖组)和高糖诱导+激动剂处理组(GW3965组)。GW3965组细胞加入10μmol/L GW3965预处理24h,之后高糖组和GW3965组细胞加入33mmol/L葡萄糖继续诱导培养24h。采用MTT法检测细胞存活率,流式细胞术检测细胞凋亡率,JC-1荧光染料法检测线粒体膜电位,Western blot检测LXRα、ERK和p38 MAPK信号通路相关蛋白的变化。结果:GW3965可以提高H9c2抑制细胞凋亡,提高细胞存活率,改善细胞线粒体膜电位变化,并抑制ERK和p38 MAPK通路活性。结论:GW3965通过介导LXRα和MAPK通路减轻高糖诱导的细胞凋亡和线粒体损伤,对心肌细胞具有保护作用。Objective： To explore the effect of GW3965 on myocardial apoptosis induced by high glucose and its mechanism.Methods： H9c2 myocardial cells were randomly divided into three groups： control group, high glucose group and GW3965 group.Cells in GW3965 group were pretreated with 10μmol/L GW3965 for 24 h.Then cultures in high glucose group and GW3965 group were induced by 33 mmol/L glucose for 24 h.MTT was used to detect cells survival.The apoptosis was detected by flow cytometry and mitochondrial membrane potential by JC-1 fluorescence staining.Western blot was used to detect the expression of proteins related to LXR, ERK and p38 MAPK signal pathway.Results： GW3965 could inhibit the apoptosis of H9c2 cells, increase the rate of cells survival and influence mitochondrial membrane potential.GW3965 inhibited the activity of ERK and p38 MAPK pathway.Conclusion： GW3965 can inhibit the apoptosis and mitochondrial damage induced by high glucose via regulating LXRα and MAPK pathway, resulting a protective effect on myocardial cells.

关 键 词：心肌 @GW3965 高血糖症 糖尿病心肌病/病理生理学 @肝X受体 细胞凋亡 

分 类 号：R392.3[医药卫生—免疫学]