荧光层析定量检测tPSA和fPSA方法研究  

Study on quantitative detection of tPSA and fPSA by fluorescent chromatography

作　　者：钱存[1] 王云龙[1,2,3] 明亮[1] 李玉林 王继创 程蕾 程春杰[3] 高玉红[3] 闫生辉[3] 

机构地区：[1]郑州大学第一附属医院,河南郑州450000 [2]河南省生物工程技术研究中心,郑州市生物制药重点实验室,河南郑州450000 [3]郑州职业技术学院,河南郑州450001

出　　处：《轻工学报》2017年第4期37-42,共6页Journal of Light Industry

基　　金：郑州市年度科技计划项目(20140820)

摘　　要：采用碳二亚胺(EDC)活化荧光微球,再将荧光微球与抗体进行偶联,制备同时定量检测总前列腺特异性抗原(t PSA)和游离前列腺特异性抗原(f PSA)的荧光层析试纸条,并进行性能分析和方法论对比.结果表明:该方法检测t PSA和f PSA的灵敏度分别为0.08 ng/m L和0.06 ng/m L;批内差异均小于10%,批间差异均小于15%;回收率均为95%~105%;特异性良好.该方法检测结果与电化学发光法相关性较好,可满足小型医院辅助诊断需要.Conjugating the EDC activated fluorescent microspheres with antibody to prepare the fluorescence chromatographic strip for simultaneous detection of t PSA and f PSA. The performance analysis and methodological comparison for the test strip were further performed. The results showed that the sensitivity for PSA and f PSA detection by the test strip were 0. 08 ng/m L and 0. 06 ng/m L,respectively. The coefficient of variation within and between groups was less than 10% and 15%,respectively. The recovery rates ranged from 95% to105%. The specificity of the test strip was well. The results revealed a good correlation between self-prepared test strip and electrochemiluminescence detection,which could satisfy the needs of aided diagnosis of smallscale hospitals.

关 键 词：TPSA FPSA 荧光微球 荧光层析 

分 类 号：R446.1[医药卫生—诊断学]