柠檬酸发酵生产中玉米原料预处理工艺的条件优化  

Optimal conditions of corn pretreatment for citric acid fermentation

作　　者：满云[1] 陶惟一 郭东升[1] 李秀娟[1] 彭钢[1] 俞亚东 黄和[3] 

机构地区：[1]南京工业大学生物与制药工程学院,江苏南京211800 [2]国家"江苏先进生物与化学制造协同创新中心",江苏南京210009 [3]南京工业大学药学院,江苏南京211800

出　　处：《南京工业大学学报（自然科学版）》2017年第4期18-25,共8页Journal of Nanjing Tech University(Natural Science Edition)

基　　金：国家自然科学基金(21506096;21476111);国家高技术研究发展计划(2013AA020302);江苏省自然科学基金(BK20131405);农业部农产品加工重点实验室开放课题(2015011);江苏高校优势学科建设工程

摘　　要：为了获得发酵生产柠檬酸中玉米粉原料预处理最佳的工艺条件,采用单因素试验和正交试验,系统分析了液化温度、液化p H、加酶量等因素对残渣淀粉含量、清液总糖含量、DE值、酶解效率等的影响。结果表明:随着液化温度的上升,DE值、残渣淀粉含量的变化呈现显著下降趋势,过滤速度呈现加快的趋势。95℃液化温度对改善DE值、过滤速度和残渣淀粉含量最有利,90℃时酶解效率最高。正交试验结果显示发酵产柠檬酸玉米原料预处理最佳条件为液化温度95℃、调浆p H 5.4、淀粉酶加量0.5 kg/t,该预处理条件能有效提高粮食利用率。In order to obtain the optimal corn powder pretreatment conditions for citric acid fermentation,single factor test and orthogonal experiments were used to analyze the effects of liquefying temperature,liquefying pH and amylase addition on residual starch content,total sugar content,dextrose equivalent（ DE） value and hydrolysis efficiency.Results indicated that liquefying temperature increment significantly decreased DE value,residual starch content and enhanced filtering rate.95 ℃ liquefying temperature was optimal for improving DE value,filtering rate and residual starch content.Hydrolysis efficiency was highest when liquefying temperature was 90 ℃. Orthogonal experiments results suggested that the optimized corn powder pretreatment conditions were 95 ℃ for liquefying,pH 5. 4 for pulp-mixing,and 0. 5 kg/t for amylase addition. The grain utilization rate could be improved significantly under the optimized pretreatment conditions.

关 键 词：柠檬酸 发酵预处理 液化温度 淀粉酶量 

分 类 号：TQ815[化学工程]