海藻提取物对病毒感染的大鼠1型糖尿病Vβ13基因表达及Treg细胞的影响(英文)  

Effects of seaweed extract on Vβ13 gene expression and Treg cells in virus-induced type 1 diabetes mellitus

作　　者：管福来[1] 尤敏[1] 孙延龙[2] 李文通[3] 王金冬[1] 吴晓燕[1] 姜子童 刘志军[1] GUAN Fu-lai YOU Min SUN Yan-long LI Wen-tong WANG Jin-dong WU Xiao-yan Jiang Zi-tong LIU Zhi-jun(Department of Microbiology Department of medicinal chemistry Department of pathology Department of Clinical Medicine, Weifang Medical UniVersity, Weifang 261053, China)

机构地区：[1]潍坊医学院病原生物学教研室,山东潍坊261053 [2]潍坊医学院药物化学教研室,山东潍坊261053 [3]潍坊医学院病理学教研室,山东潍坊261053 [4]潍坊医学院临床医学院,山东潍坊261053

出　　处：《中国海洋药物》2017年第3期34-40,共7页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金项目(81471048);山东省自然科学基金项目(ZR2012HL42,2015ZRB14132);山东省教育厅高等学校科技计划项目(J12LK01);潍坊市科学技术发展计划项目(2014WS040)资助

摘　　要：目的观察Vβ13mRNA和Treg细胞在大鼠细小病毒(KRV)联合巨细胞病毒(CMV)感染大鼠的脾淋巴细胞中的表达以及海藻提取物对1型糖尿病的治疗效果。方法 LEW.1WR1大鼠40只,随机分成2组,应用CMV联合KRV建立大鼠1型糖尿病模型。治疗组大鼠每日口服海藻提取物共40d;成模组用等量无菌生理盐水替代同步进行。RT-PCR检测脾脏Vβ13mRNA的表达,流式细胞术检测脾细胞Vβ13^+CD4^+CD25^+Treg细胞亚群比例;HE染色观察糖尿病胰岛的病变情况。结果实验组成模率为90%(18/20),成模血糖平均水平在3.15g/L;实验组Vβ13的表达高于治疗组。实验组中CD4^+CD25^+Treg细胞的比例为6.2%,治疗组为13.5%(P<0.05)。结论感染巨细胞病毒的大鼠Vβ13 mRNA的表达在糖尿病初期显著增高。Vβ13^+CD4^+CD25^+Treg细胞亚群比例较低。海藻提取物提高了LEW.1WR1大鼠Treg细胞的表达,可用于治疗糖尿病。Objective To measure the expression of Vβ13 mRNA and Treg cells in spleen lymphocytes from rats infected with cytomegalovirus（CMV）as well as Kilham rat virus（KRV）and the therapeutic effects of seaweed extract on type 1diabetes mellitus.Methods Forty LEW.1WR1 rats were randomly divided into two groups.The rat model of type 1diabetes mellitus was established using rat cytomegalovirus combined with KRV.The rats in the treatment group were treated with seaweed extract daily for 40 days,while the experimental group was given the same amount of sterile saline.The expression of Vβ13mRNA in the spleen was assessed by RT-PCR and CD4~＋CD25~＋Treg cells were detected by flow cytometry（FCM）.The sections in splenic cells were detected by HE staining.Results In the experimental group,90% of the rats were diabetic（18/20）,and the mean blood glucose level was 3.15g·L^-1.The expression of Vβ13in the experimental group was higher than that in the treatment group.The ratio of CD25~＋Treg cells in the experimental group was 6.2% and in the treatment group 13.5%（P0.05）.Conclusion The expression of Vβ13mRNA was significantly higher in the early stage of virus-induced diabetes,and the proportion of CD4~＋CD25~＋Treg cells was lower in the experimental group.Seaweed extract increased the Treg cell expression for the treatment of diabetes.

关 键 词：巨细胞病毒 1型糖尿病 海藻提取物 Vβ13 TREG细胞 

分 类 号：R965[医药卫生—药理学]