Biodegradation of 3,5-dimethyl-2,4-dichlorophenol in saline wastewater by newly isolated Penicillium sp.yz11-22N2  

作　　者：Zhou Yan Huijun He Chunping Yang Guangming Zeng Le Luo Panpan Jiao Huiru Li Li Lu 

机构地区：[1]College of Environmental Science and Engineering,Hunan University,Changsha 410082,China [2]Key Laboratory of Environmental Biology and Pollution Control,Hunan University,Ministry of Education,Changsha 410082,China [3]Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling,College of Environmental Science and Engineering,Zhejiang Gonqshang University,Hangzhou,Zhejiang 310018,China

出　　处：《Journal of Environmental Sciences》2017年第7期211-220,共10页环境科学学报（英文版）

基　　金：supported by the International S&T Cooperation Program of China(No.2015DFG92750);the National Natural Science Foundation of China(Nos.51478172 and 51278464);the Natural Science Foundation of Zhejiang Province of China(No.LY17E080002)

摘　　要：In this study,the performance of 3,5-dimethyl-2,4-dichlorophenol（DCMX） degradation by a screened strain was investigated.18 S r DNA and the neighbor-joining method were used for identification of the isolated strain.The results of phylogenetic analysis and scanning electron micrographs showed that the most probable identity of the screened strain should be Penicillium sp.Growth characteristics of Penicillium sp.and degradation processes of DCMX were examined.Fourier transform infrared spectroscopy of the inoculated DCMX solution was recorded,which supported the capacity of DCMX degradation by the screened Penicillium sp.Under different salinity conditions,the highest growth rate and removal efficiency for DCMX were obtained at p H 6.0.The removal efficiency decreased from 100%to 66% when the DCMX concentration increased from 5 to 60 mg/L,respectively.Using a Box–Behnken design,the maximum DCMX removal efficiency was determined to be 98.4%.With acclimation to salinity,higher removal efficiency could be achieved.The results demonstrate that the screened Penicillium sp.has the capability for degradation of DCMX.In this study,the performance of 3,5-dimethyl-2,4-dichlorophenol（DCMX） degradation by a screened strain was investigated.18 S r DNA and the neighbor-joining method were used for identification of the isolated strain.The results of phylogenetic analysis and scanning electron micrographs showed that the most probable identity of the screened strain should be Penicillium sp.Growth characteristics of Penicillium sp.and degradation processes of DCMX were examined.Fourier transform infrared spectroscopy of the inoculated DCMX solution was recorded,which supported the capacity of DCMX degradation by the screened Penicillium sp.Under different salinity conditions,the highest growth rate and removal efficiency for DCMX were obtained at p H 6.0.The removal efficiency decreased from 100%to 66% when the DCMX concentration increased from 5 to 60 mg/L,respectively.Using a Box–Behnken design,the maximum DCMX removal efficiency was determined to be 98.4%.With acclimation to salinity,higher removal efficiency could be achieved.The results demonstrate that the screened Penicillium sp.has the capability for degradation of DCMX.

关 键 词：Saline wastewater 3 5-Dimethyl-2 4-dichlorophenol Penicillium sp. Laccase 

分 类 号：X703[环境科学与工程—环境工程]