应用PCR方法快速鉴定SPF金定鸭性别  

Application of PCR Method for Rapid Sex Determination in SPF Jinding Duck

作　　者：徐丽晶[1,2] 孙畅[1] 陆涛峰[1] 陈洪岩[1] 赵丽丽[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室/黑龙江省实验动物与比较医学重点实验室,实验动物与比较医学创新团队,哈尔滨150069 [2]东北农业大学生命科学学院,哈尔滨150030

出　　处：《实验动物与比较医学》2017年第3期249-251,共3页Laboratory Animal and Comparative Medicine

基　　金：国家科技支撑计划(2015BAI07B02-02);中国农业科学院基本科研业务费专项(Y2016PT41);黑龙江省自然科学基金重点项目(ZD2016006)

摘　　要：目的利用PCR方法鉴定SPF金定鸭的性别。方法根据已有报道合成一对禽类性染色体上的染色质解螺旋蛋白DNA结合蛋白1(CHD1)基因的通用引物gCHD。采集培育中的85只SPF金定鸭血液样品,提取血液基因组DNA,进行PCR扩增。通过分析PCR扩增产物凝胶电泳后的条带数判定禽类性别。结果 PCR扩增产物经琼脂糖电泳分析后,雌性鸭样品扩增出CHD1-Z和CHD1-W,而雄性鸭样品只出现CHD1-Z。结论本文建立的方法可作为禽类性别的有效鉴定方法。Objective To determine the sex of SPF Jinding Duck, the universal primer gCHD of avian chromosomal DNA-binding protein 1 （CHD1） gene on avian chromosomes were synthesized. Methods Blood of 85 SPF Jinding Duck were collected and genomic DNA was extracted for PCR amplification. The sex of the birds were determined by analyzing the number of bands after gel electrophoresis. Results The CHD1-Z and CHD1-W were found in the PCR products of agarose gel electrophoresis from females and CHD1-Z was from males. Conclusions The PCR can be used as an effective method for sex identification in birds.

关 键 词：SPF金定鸭 性别鉴定 PCR 

分 类 号：S834[农业科学—畜牧学]