人参锈腐病菌拮抗菌的筛选、鉴定及发酵条件优化  

Screening,Identification and Optimization of Fermentation Condition of an Antagonistic Bacteria Against Ginseng Rusted Root Caused by Cylindrocarpon destructans

作　　者：邓进超 关一鸣[1] 吴连举[1] 张亚玉[1] 

机构地区：[1]中国农业科学院特产研究所,长春130112

出　　处：《东北农业科学》2017年第3期31-38,共8页Journal of Northeast Agricultural Sciences

基　　金：吉林省科技厅重点科技攻关项目(20140204056YY)

摘　　要：从健康人参根际土壤中分离获得一株生防菌株ATB4。根据16S rDNA、gyrA基因序列分析,结合形态学、生理与生化特性鉴定,将生防菌株ATB4鉴定为甲基营养型芽孢杆菌(Bacillus methylotrophicus)。通过单因素试验和正交试验设计,对其发酵培养基成分培养条件进行优化,探究生防菌对引起人参锈腐病的Cylindrocarpon destructans防治效果,最终得到该菌最适培养基成分为3%葡萄糖、4%酵母浸粉、0.05%MnSO_4·7H_2O和0.1%K_2HPO_4,最佳发酵条件为4%接菌量,初始pH 8.0、培养温度28℃、培养24 h、250 mL三角瓶装液量40 mL。One antagonistic bacterial strain ATB4 was isolated from the rhizosphere soil samples of healthy ginseng.Based on phylogenetic analyses of 16S rDNA and gyrA gene sequences, and morphological, physiological, biochemi-cal characterizations, the strain ATB4 was identified as Bacillus methylotrophicus. The single factor experiment andorthogonal experiment were adopted to optimize the liquid fermentation medium and conditions that enhance its bio-control efficacy against Cylindrocarpon destructans caused ginseng rusted root. The best compositions of cultural me-dium were as follow： 3% glucose, 4% yeast extract powder, 0.05% Mn SO4·7H2O and 0.1% K2HPO4. The optimal fer-mentation conditions were as follow： inoculation rate 4%, initial pH 8.0, temperature 28℃, fermentation time 24 h,and medium capacity 40 mL/250 mL.

关 键 词：人参锈腐病 生防菌 发酵条件优化 

分 类 号：S435.675[农业科学—农业昆虫与害虫防治]