Determination of Pathogenicity and Crude Toxin Activity of Rhizoctonia solani with Rice Tissue In Vitro  

作　　者：Ren Shaofeng Dong Wenhan Liu Lie Yue Rui Li Chengyun Yang Genhua 

机构地区：[1]Key Laboratory of Agro-Biodiversity and Pest Management,the Ministry of Education,Yunnan Agricultural University [2]Science and Technology Bureau of Yunnan Agricultural University

出　　处：《Plant Diseases and Pests》2017年第3期6-11,28,共7页植物病虫害研究（英文版）

基　　金：Supported by National Natural Science Foundation of China(31160352,31360423);Science and Technology Innovation Team Support Plan for Colleges and Universities in Yunnan Province(YJK[2014]22)

摘　　要：[ Objectives] As a traditional inoculation method of rice sheath blight, rice relative lesion height method needs rice grow to late tillering or heading stage and then inoculate in vivo, which is time-consuming and is difficult to control the testing conditions, so it is urgent to search a better alternative method. [ Methods] With rice relative lesion height method （ in vivo inoculation） as the control, the pathogenicity and crude toxin activity of Rhizoctonia solani were determined by rice detached leaf sheath method and rice detached leaf method, to compare the correlation of in vivo and in vitro inoculation. [Results] Rice detached leaf method and rice detached leaf sheath method had significantly positive correlation with rice relative lesion height method, and the determination results of rice detached leaf method and rice detached leaf sheath method could reflect the pathogenicity and crude toxin activity of R. solani accurately. [ Conclusions] Rice detached leaf method and rice detached leaf sheath method are easy to operate and control testing conditions, and could be used as alternative methods to determine pathogenicity and etude toxin activity of R. solani.[ Objectives] As a traditional inoculation method of rice sheath blight, rice relative lesion height method needs rice grow to late tillering or heading stage and then inoculate in vivo, which is time-consuming and is difficult to control the testing conditions, so it is urgent to search a better alternative method. [ Methods] With rice relative lesion height method （ in vivo inoculation） as the control, the pathogenicity and crude toxin activity of Rhizoctonia solani were determined by rice detached leaf sheath method and rice detached leaf method, to compare the correlation of in vivo and in vitro inoculation. [Results] Rice detached leaf method and rice detached leaf sheath method had significantly positive correlation with rice relative lesion height method, and the determination results of rice detached leaf method and rice detached leaf sheath method could reflect the pathogenicity and crude toxin activity of R. solani accurately. [ Conclusions] Rice detached leaf method and rice detached leaf sheath method are easy to operate and control testing conditions, and could be used as alternative methods to determine pathogenicity and etude toxin activity of R. solani.

关 键 词：Rhizoctonia solani PATHOGENICITY Toxin activity 

分 类 号：S435.111.42[农业科学—农业昆虫与害虫防治]