多粘菌素E高产菌株的选育  

Mutation breeding of high colistin producing strains

作　　者：邢维玲[1] 周希贵[1] 王贺祥[1] 肖希龙[1] 宋渊[1] 章红[1] 

机构地区：[1]中国农业大学生物学院,北京100094

出　　处：《中国抗生素杂志》2002年第6期326-327,353,共3页Chinese Journal of Antibiotics

摘　　要：以多粘类芽胞杆菌 (Paenibacilluspolymyxa) 18 3为出发菌株 ,采用紫外线诱变 ,并以重金属Cu2 +作为筛选因子 ,获得一株遗传稳定且多粘菌素E发酵效价比出发菌株提高 45 %的菌株。对该菌株再采用亚硝基胍 (NTG)诱变 ,以缬氨酸结构类似物α 氨基丁酸作为筛选因子时得到一株产量提高 3A colistin producing strain Paenibacillus polymyxa 18 3 was treated by UV and high concentration of heavy metal Cu 2+ as screening factor, a stable strain was obtained with the titer of colistin increased by 45% than parent strain. Then the mutant which grow on media containing α amino butyric acid (an analogue of valine) was treated with NTG, the productivity was increased by 34%.

关 键 词：多粘菌素E 亚硝基胍诱变 紫外线诱变 重金属Cu^2+ α-氨基丁酸 碱性多肽类抗生素 

分 类 号：TQ465.6[化学工程—制药化工] Q933[生物学—微生物学]