六价铬对职业人群细胞周期相关基因表达的影响  

A study on the mRNA expression of cell cycle related genes in population occupationally exposed to hexavalent chromium

作　　者：郭心念 童延 贾君麟 王海明[2] 冯玲芳[1] 夏海玲[1] 蒋兆强[1] 楼建林[1] 

机构地区：[1]浙江省医学科学院职业病防治研究所,浙江杭州310000 [2]奉化市疾病预防控制中心

出　　处：《预防医学》2017年第7期670-674,共5页CHINA PREVENTIVE MEDICINE JOURNAL

基　　金：国家自然科学基金(81472960;81001242);浙江省自然科学基金(LY13H260002);浙江省科技计划(2015F10013)

摘　　要：目的研究六价铬暴露对职业人群细胞周期相关基因表达的影响。方法选取某电镀厂155名六价铬职业暴露工人为研究对象,调查工人基本情况并采集肝素抗凝外周血,采用电感耦合等离子体质谱仪检测血铬水平(ppb),根据血铬水平将研究对象分为4组(组1≤0.04 ppb、0.04 ppb<组2≤1 ppb、1 ppb<组3≤5 ppb、组4>5 ppb),比较各组p16和CDK6基因表达水平,并分析基因表达的影响因素。结果共纳入155名工人,其中男性89人,女性66人,平均(39.65±8.86)岁,工龄中位数为36个月,血铬中位数为0.95 ppb。4组p16基因表达水平中位数(四分位间距)分别为4.22(19.91)、7.19(30.71)、7.47(21.47)和14.60(27.28),组间差异无统计学意义(P=0.19)。4组CDK6基因表达水平中位数(四分位间距)分别为4.00(8.13)、15.05(32.72)、8.03(17.32)和24.81(35.09),其中组1的CDK6基因表达水平低于其他各组(P<0.01)。Spearman相关分析结果显示,CDK6基因表达水平与血铬水平呈正相关(rs=0.28,P<0.01);Logistic回归分析结果显示,血铬水平高是CDK6基因过量表达的危险因素(OR=1.168,95%CI:1.040~1.312)。结论六价铬职业暴露可致细胞周期相关基因CDK6表达升高。Objective The aim of our study was to investigate the influence of hexavalent chromium exposure on mRNA expression of cell cycle related genes in electroplating workers, and to provide population data for investigating the toxic mechanisms of hexavalent chromium. Methods A total of 155 cases of workers occupationally exposed to hexavalent chromium were selected, including 89 males and 66 females, and the average age of workers was 39.65＆#177;8.856 years old. Questionnaire was used to collect essential information of workers. Peripheral blood was collected from electroplating workers. The inductively couple plasma mass spectrometry （ICP-MAS） was used to measure total blood chromium content. The workers were divided into four groups according to the blood chromium content. After extracting total RNA from whole blood and reverse transcription, the mRNA expression levels of p16 and CDK6 genes were detected by real-time quantitative PCR. Meanwhile, the levels of blood chromium （BCr） and the mRNA expression of p16 and CDK6 genes were compared among four groups. The impact of BCr, smoking habits, drinking habits, gender on the mRNA expression of CDK6 gene was analyzed. Results The levels of BCr in group 1 to 4 were 0.04ppb, 0.47＆#177;0.29 ppb, 2.76＆#177;1.16 ppb, 9.36 ＆#177;4.38 ppb, respectively, and the difference between every two groups was significant （P〈0.05） . The median of p16 gene expression in four groups was 4.22, 7.19, 7.47, and 14.60, respectively, and the difference between every two groups was not significant （P〉0.05） . The mRNA expression levels of CDK6 gene in groups 2 to 4 were 15.05, 8.03 and 24.81, respectively, which were significantly higher than that in group 1 （P〈0.05） . The results of logistic regression showed that the level of Bcr was the main influence factor, while smoking habits, drinking habits and gender had no obvious impact on the mRNA expression of CDK6 gene. Conclusions Long-term exposure of hexavalent chromium led to higher mRNA expression of CDK6 ge

关 键 词：六价铬 职业暴露 细胞周期 CDK6基因 P16基因 

分 类 号：R114[医药卫生—卫生毒理学]