不同稀释液在乙型肝炎病毒DNA稀释检测中的应用研究  

The application of evaluation of the different diluents used in the HBV-DNA diluted detection

作　　者：汪东剑[1] 彭慧琼[1] 彭国林[1] 林萍[1] 徐波[1] 刘仙[1] WANG Dongjian PENG Huiqiong PENG Guolin L IN Ping XU Bo L IU Xian(No. 184 Hospital of People＇s Liberation Army ,Yingtan ,Jiangxi 335000 ,China)

机构地区：[1]中国人民解放军第一八四医院,江西鹰潭335000

出　　处：《检验医学与临床》2017年第13期1864-1866,共3页Laboratory Medicine and Clinic

基　　金：南京军区医学科技创新课题项目(MS078)

摘　　要：目的分析和评价使用不同稀释液对高载量标本进行乙型肝炎病毒(HBV)-DNA稀释检测的结果,评估临床常用稀释液在高载量标本HBV-DNA稀释检测中的应用价值。方法收集初次检测HBV-DNA载量超出线性范围的标本30份,比较使用阴性质控品、阴性血清、生理盐水、蒸馏水作为稀释液进行稀释检测结果的差异。结果高载量标本进行稀释检测时,使用阴性血清、生理盐水、蒸馏水进行稀释,其检测结果与使用阴性质控品作为稀释液时的结果比较,差异无统计学意义(F=1.198,P=0.314);偏差均在允许总误差范围内,但偏差之间的差异有统计学意义(F=16.945,P<0.05)。使用蒸馏水作为稀释液时偏差最小,且该结果与使用阴性质控品进行稀释检测的结果呈良好的线性关系,回归方程为Y=0.970 X+0.255(Y=阴性质控品稀释检测结果,X=蒸馏水稀释检测结果),R^2=0.959。结论当试剂盒自带阴性质控品不能满足高载量标本HBV-DNA稀释检测需要时,使用蒸馏水作为稀释液进行稀释检测能取得较满意的结果。Objective Analysis and evaluation the results of HBV DNA diluted detection using different diluents,to evaluation the application value of those diluents in diluted detection of high loads sample. Methods 30 samples which the first detection of HBV-DNA loads exceed the linear scope were collected, compared the difference between results when use negative quality product, negative serum,physiological saline, distilled water as diluents. Results In time of diluted detection of those high loads sample, there was no significant difference between the results（F= 1. 198,P=0. 314） when use negative quality product, negative serum, physiological saline,distilled water as diluents;the TEa were within the allowed range,but the difference between bias was statistically significant（F= 16. 945 ,P〈0. 05） ;the bias was minimum in time of use distilled water as diluents,and the results of distilled water diluted detection had good linear relationship between that of negative quality product diluted detection, the fitting equation was Y=0. 970X＋0. 255（Y results of negative quality product diluted detection, X= results of distilled water diluted detection）, R2 =0. 959. Conclusion In time of negative quality product could not meet the need of diluted detection,using distilled water as diluents could achieve satisfactory results.

关 键 词：稀释液 乙型肝炎病毒 脱氧核糖核酸 

分 类 号：R512.62[医药卫生—内科学]