蛋鸡心包积水综合征病原鉴定及组织灭活疫苗紧急免疫试验  

Pathogen Identification of Hydropericardium Syndrome and Contingency Vaccination with A Developed Tissue Inactivated Vaccine in Breeding Hens

作　　者：刘吉山[1,2] 肖跃强[2] 褚仁忠 于雪 姚春阳[2] 苗立中[2] 杨慧 柳增善[1] 沈志强[2] 

机构地区：[1]吉林大学动物医学学院,吉林长春130062 [2]山东省滨州畜牧兽医研究院,山东滨州256600 [3]阳信县畜牧兽医局,山东阳信251800 [4]山东绿都生物科技有限公司,山东滨州256600

出　　处：《动物医学进展》2017年第7期37-41,共5页Progress In Veterinary Medicine

基　　金：山东省现代农业产业技术体系家禽创新团队项目(SDAIT-11-16)

摘　　要：2016年4月,山东蛋鸡养殖规模25 000余只的鸡场发生了一种高致死率传染病,病死率15%以上。通过临床症状观察、病理剖检、血凝试验、动物回归试验、PCR检测及血清型分析,确诊该病为禽腺病毒Ⅰ群4型感染所致。无菌采集该养殖场病死鸡肝脏,制备组织灭活疫苗,对鸡群进行紧急注射免疫,免疫接种后7d,病死鸡数量开始逐渐下降,12d后完全停止发病死亡,最终成功控制了该病。应用制备的同源组织灭活疫苗紧急免疫鸡群有效控制了禽腺病毒Ⅰ群4型的感染,为该病提供了一种可靠的防控措施。In april 2016,a highly lethal infectious disease emerged in a farm of more than 25 000 breeding hens locating in Shandong province, and the mortality rate reached to 15%, which resulted in heavy economic losses.The disease was diagnosed through the clinical symptoms, autopsy lesions, hemagglutination test,PCR detection,serotype analysis anti animal regression tests.The results showed that the fowl adenovirus type 4 （FAdV-4） was the agent of this disease.The livers of sick or dead chickens were collected and the tissue inactivated vaccine was developed, and all animals in this farm were performed contingency vaccination, 7 days later,the number of sick hens began to decrease gradually,and there were no more infected animals at 12 day time point,so the disease was successfully controlled through the tissue inactivated vaccine immunization.This study provided a reliable measure for prevention and control of the epidemic disease with FAdV-4 infection through developing the homologous tissue inactivated vaccine and thereafter the contingency vaccination.

关 键 词：心包积水综合征 蛋鸡 禽腺病毒Ⅰ群4型 组织灭活疫苗 紧急免疫 

分 类 号：S852.659.1[农业科学—基础兽医学] S858.31[农业科学—兽医学]