不同低温海水浸泡对清醒大鼠生存时间及死亡率的实验研究  

Experimental study on survival time and mortality of conscious rats immersed in different cold seawater

作　　者：陈丽娜[1] 奂剑波 史成和[3] 代维[4] 袁丹凤[4] 周继红[4] 韩志海[1] 

机构地区：[1]海军总医院呼吸与危重症医学科,安徽医科大学海军临床学院,北京100048 [2]第二军医大学海军临床学院,北京100048 [3]海军总医院海战伤救治研究中心,北京100048 [4]第三军医大学大坪医院野战外科研究所第四研究室,重庆400042

出　　处：《局解手术学杂志》2017年第7期469-474,共6页Journal of Regional Anatomy and Operative Surgery

基　　金：海军应用基础研究重点项目(BHJ14C009);海军应用基础研究面上项目(CWS12J012)

摘　　要：目的观察不同温度海水浸泡对清醒大鼠生理状态和生存时间及死亡率的影响。方法 100只SD雄性大鼠(腹腔预置温度传感器),随机分为5组,分别为20℃海水浸泡组、17℃海水浸泡组、15℃海水浸泡组、13℃海水浸泡组、10℃海水浸泡组,每组20只,观察和记录5组大鼠海水浸泡2 h内呼吸、心率及肌颤变化,记录浸泡24 h内每只大鼠的生存时间及计算各组的死亡率,回顾性分析浸泡2 h内各组大鼠腹腔温度的下降趋势。结果浸泡10 min,各组大鼠的呼吸、心率均明显上升,但此时各组之间差异没有统计学意义(P>0.05);10~40 min各组大鼠呼吸和心率迅速下降,40~80 min下降相对较缓,浸泡80 min,各组大鼠呼吸有显著性差异(P<0.05);浸泡100 min,各组间大鼠心率有显著差异(P<0.05)。浸泡20 min,15℃组、13℃组、10℃组肌颤达高峰,各组之间有明显差异(P<0.05),随后肌颤消失;浸泡30 min,20℃组与17℃组肌颤达高峰,但2组之间无明显差异(P>0.05),随后肌颤维持在一定水平。浸泡24 h内,20℃组、17℃组、15℃组、13℃组及10℃组大鼠的平均存活时间分别为(23.6±1.23)h、(15.0±4.16)h、(7.7±3.21)h、(2.4±0.91)h、(1.1±0.39)h,组间差异均有统计学意义(P<0.05)。各大鼠腹腔温度均呈断崖式下降,水温越低,下降越快,浸泡40 min后,各组大鼠腹腔温度的差异有统计学意义(P<0.05)。结论不同温度海水浸泡对大鼠的生理状态和生存时间及死亡率产生明显差异,水温越低,生理状态变化越明显,存活时间越短,死亡率越高。Objective To observe the effects of seawater immersion at different temperatures on survival time and mortality and physio- logical state of non-anesthetized rats. Methods Totally 100 SD male rats（The abdominal cavity was implanted with a temperature sensor in advance）were randomly divided into five groups which were immersed in 20℃ ,17 ℃ ,15 ℃ ,13 ℃ and 10 ℃ seawater,respectively. Each group contains 20 rats. The changes of respiration, heart rate and muscle fibrillation within 2 hours were observed and the survival time and mortality of each group were counted in 24 hours. The decrease trend of intraperitoneal temperature in rats was analyzed retrospectively within 2 hours. Results Soaking for 10 minutes,the respiratory and heart rate of each group were significantly increased,but there was no signifi- cant difference among groups（ P 〉 0.05 ）. The respiratory and heart rate decreased rapidly between 10 and 40 minutes, and the decline was slower relatively between 40 and 80 minutes. Soaking for 80 minutes,the respiration rate of rats among groups had significant difference （P 〈 0. 05 ）. Immersing for 100 minutes, the heart rates of rats in each group were significantly different （ P 〈 0.05 ）. Soaking for 20 minutes, the muscle fi- brillation of 15 ℃, 13 ℃ and 10 ℃ group reached the peak, and there were significant differences among groups （P 〈 0.05 ） , then disap- peared. However the rats in 20 ℃ and 17 ℃ group reached the peak at 30 minutes, but there was no significant difference between two groups （ P 〉 0.05 ） , hereafter the muscle fibrillation was maintained at a certain level. The mean survival time of 20℃ group, 17 ℃ group, 15 ℃ group, 13 ℃ group and 10 ℃ group in 24 hours were （ 23.6 ± 1.23 ） hours, （ 15.0 ± 4.16 ） hours, （ 7.7 ± 3.21 ） hours, （ 2.4 ± 0.91 ） hours and （ 1.1 ± 0.39） hours, respectively, and the survival curve of each group was statistically significant （ P 〈 0.05 ）. The intraperitoneal temperatu

关 键 词：海水浸泡 大鼠 死亡率 生存时间 体温过低症 

分 类 号：R339.6[医药卫生—人体生理学]