术中扩张对大隐静脉桥血管狭窄相关基因表达的影响  

Effects of intraoperative expansion on expression of stenosis-related genes in saphenous vein graft

作　　者：庞磊[1] 孟镔[2] 李曦哲 施盛[1] 叶一舟[1] 

机构地区：[1]上海交通大学附属第一人民医院心脏及大血管外科,上海200080 [2]安徽理工大学医学院临床外科应用解剖教研室,安徽淮南232001

出　　处：《局解手术学杂志》2017年第7期474-478,共5页Journal of Regional Anatomy and Operative Surgery

基　　金：上海市科委生药处引导项目(114119b1600)

摘　　要：目的探讨冠状动脉旁路移植术(CABG)中高压扩张对人体大隐静脉桥血管(SVG)狭窄相关基因VE-cad、Egr-1、Versican表达的影响。方法收集扩张和未扩张的大隐静脉标本各10例分为扩张组和未扩张组,利用组织实时荧光定量PCR(RTPCR)和免疫组化检测VE-cad、Egr-1、VCAN基因mRNA及蛋白表达水平,分析VE-cad、Egr-1、VCAN基因mRNA及蛋白表达水平情况。采用Student’s t检验和χ~2检验或Fisher确切概率法进行统计学分析。结果 RT-PCR结果示:扩张组Egr-1、VCAN基因转录的mRNA明显高于未扩张组,扩张组VE-cad转录的mRNA明显低于未扩张组。免疫组化染色结果显示:扩张组Egr-1、VCAN表达显著强于未扩张组,扩张组VE-cad表达显著弱于未扩张组。结论 CABG术中大隐静脉桥血管高压扩张可导致桥血管狭窄相关基因Egr-1、VCAN的表达升高,VE-cad基因的表达降低,可能与CABG术后大隐静脉桥血管再狭窄有关。Objective To investigate the effect of high pressure distention on the expression of stenosis-related genes of saphenous win graft（SVG） during the coronary artery bypass grafting（CABG）. Methods The biopsy specimens of saphenous vein collected from 10 patients who have undergone CABG,were divided into expansion group and no-expansion group. Real-time PCR and immunohistochemical stai- ning were performed for examination of mRNA and protein expression of VE-cad,Egr-I ,VCAN respectively. Student＇ s t and Chi-square test were used to do statistic analysis. Results The results of RT-PCR showed that the mRNA transcription of Egr-1, VCAN in the expansion group were statistically significantly higher than those in no-expansion group（ P 〈0. 05 ）. The mRNA transcription VE-cad in expansion group was statistically significantly lower than that in the no-expansion group （ P 〈 0.05 ）. The immunohistochemical staining results showed that the expression of Egr-1 and VCAN in expansion group were significantly stronger than those in no-expansion group, while the expression of VE- cad was significantly lower than no-expansion group. Conclusion The intraoperative expansion of SVG can increase the expression of steno- sis-related genes Egr-1 and Versican, and decrease the expression of stenosis-related gene VE-cad, which may be related with the SVG stenosis after CABG.

关 键 词：冠状动脉旁路移植术 VE-cad EGR-1 VERSICAN 大隐静脉桥血管 高压扩张 

分 类 号：R654[医药卫生—外科学]