AGEs/RAGE/SphK1信号通路介导马钱苷抗AGEs损伤GMCs的机制研究  

Study on Mechanism of Loganin Against AGEs Induced GMCs Injury via AGEs/RAGE/SphK1 Pathway

作　　者：吕兴[1,2] 许惠琴[1,2] 吕高虹[1,2] 吴云皓[1,2] 陈玉萍[1,2] 沈红胜 戴国英[1,2] 徐康[1,2] LYU Xing XU Hui-qin LYU Gao-hong VCU Yun-hao CHEN Yu-ping SHEN Hong-sheng DAI Guo- ying XU Kang~＇(l. School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China Jiangsu Provincial Key Laboratory of Chinese Medicine Efficacy and Safety Evaluation, Nanjing, 210023, China)

机构地区：[1]南京中医药大学药学院,江苏南京210023 [2]江苏省中药药效与安全性评价重点实验室,江苏南京210023

出　　处：《南京中医药大学学报》2017年第4期382-385,共4页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家自然科学基金(81073111);江苏省普通高校研究生科研创新计划项目(CXZZ13_0622);江苏高校优势学科建设工程资助项目(Nzyzyxjg-1006)

摘　　要：目的探讨山茱萸中有效成分马钱苷在缓解晚期糖基化终末产物(AGEs)诱导肾小球系膜细胞(GMCs)损伤的机制。方法体外培养GMCs,分为空白对照组,模型组(AGEs:200mg/L),马钱苷0.1、1、10μmol/L浓度组。检测细胞增殖率、细胞FN及COL-Ⅳ分泌水平,观察细胞微观结构变化,测定RAGE、SphK1、S1P和TGF-β蛋白表达量。结果马钱苷可有效抑制AGEs诱导的GMCs增殖,减少FN及COL-Ⅳ分泌,缓解细胞微观结构病变,下调RAGE、SphK1、S1P和TGF-β蛋白表达。结论马钱苷通过下调AGEs/RAGE/SphK1信号通路,缓解AGEs诱导的GMCs损伤,从而改善糖尿病肾病(DN)。OBJECTIVE To study the mechanism of loganin, the effective componet of Cornus officinalis, on protecting the glomerulus mesangial cell （GMCs） injured by advanced glycation end products （AGEs）. METHODS GMCs were divided into control, model （AGEs： 200 mg/L）, loganin （0.1, 1, 10μmol/L） groups. The cell proliferation, FN and COL-Ⅳ secretion, microstructure lesions, RAGE, SphK1, S1P and TGF-13 protein expressions were measured. RESULTS AGEs induced cell proliferation, FN and COL-Ⅳ secretions, microstructure lesions, RAGE, SphK1, S1P and TGF-β protein expressions were suppressed by loganin. CONCLUSION Loganin can improve the AGEs induced GMCs injury by regulating the AGEs/RAGE/ SphK1 signal pathway, and then ameliorate the diabetic nephropathy.

关 键 词：山茱萸 马钱苷 晚期糖基化终末产物 肾小球系膜细胞 糖尿病肾病 

分 类 号：R285.5[医药卫生—中药学]