过敏性紫癜患儿粪便细菌代谢相关产物短链脂肪酸水平测定及其意义  

Determination of the level of fecal short-chain fatty acids of bacterial associated metabolites in children with Henoch-Schonlein purpura and its clinical value

作　　者：胡红卫[1] 潘媛 赵波[3] 刘梅[1] 熊晶晶[1] 廖亚彬[3] 吉晓菲[1] 张婷婷[1] 张丽芝[1] 黄永坤[1] 

机构地区：[1]昆明医科大学第一附属医院儿科,云南昆明650032 [2]云南省产品质量监督检验研究院,云南昆明650221 [3]昆明医科大学附属儿童医院风湿免疫科,云南昆明650228

出　　处：《中国实用儿科杂志》2017年第7期531-533,共3页Chinese Journal of Practical Pediatrics

基　　金：国家自然科学基金项目(81360068);云南省自然科学基金面上项目(2013FB137);"十二五"云南省特色学科建设项目(201109)

摘　　要：目的测定过敏性紫癜(HSP)患儿粪便中细菌代谢相关产物短链脂肪酸水平并研究其意义。方法选取2014年6月至2015年1月昆明医科大学第一附属医院儿科确诊为HSP 27例患儿,其中男15例、女12例;平均年龄(9.0±2.8)岁。均符合中华医学会儿科学分会免疫学组推荐的HSP诊断标准。健康儿童28例为对照组,其中男16例,女12例;平均年龄(9.5±1.3)岁。收集保存HSP患儿急性期、恢复期和对照组的粪便标本。应用气相色谱技术定量测定对照组、HSP急性期组和恢复期组粪便中短链脂肪酸包括乙酸、丙酸、丁酸、异丁酸、异戊酸浓度。结果对照组、HSP急性期组和恢复期组粪便乙酸浓度(mol/g)分别为(6.04±2.15)×10^(-5)、(3.90±3.08)×10^(-5)和(4.73±3.95)×10^(-5),对照组与急性期组比较,对照组高于急性期组且差异有统计学意义(P<0.05),而与恢复期组比较,差异无统计学意义(P>0.05)。丁酸浓度分别为[4.53(2.68,5.47)]×10^(-5)、[1.48(0.14,3.78)]×10^(-5)和[0.93(0.31,1.75)]×10^(-5),对照组高于急性期组及恢复期,且均有统计学意义(P<0.017)。丙酸浓度分别为[3.22(2.22,4.20)]×10^(-5)、[1.80(7.84,3.66)]×10^(-5)和[2.68(0.46,4.93)]×10^(-5),组间差异无统计学意义(P>0.017)。异丁酸浓度分别为[0.19(0.07,0.30)]×10^(-5)、[0.17(0.08,0.59)]×10^(-5)和[0.14(0.03,0.30)]×10^(-5),异戊酸浓度分别为[0.13(0.04,0.19)]×10^(-5)、[0.13(0.06,0.32)]×10^(-5)和[0.09(0.02,0.23)]×10^(-5),组间差异无统计学意义(P>0.017)。结论 HSP急性期患儿粪便乙酸、丁酸显著降低。恢复期粪便乙酸虽有升高,但仍未完全回到正常水平,而粪便中丁酸浓度更低。Objective To determine the level of fecal short-chain fatty acids （SCFA） of bacterial associated metabolites in children with Henoch-Schonlein purpura（HSP） and study its clinical value. Methods From June 2014 to January 2015, twenty-seven children with HSP and twenty-eight healthy children were enrollded in this study. The fecal samples were collected respectively in the acute phase and in the remission phase. SCFA were extracted from children＇ s feces, and quantitatively analyzed by gas chromatography （GC）. Results The content of acetic acid in control subjects （6.04±2.15）×10^-5 was significantly higher than that in active stage of patients with HSP[ （3.90±3.08）×10^-5, P 〈 0.05], while there were no significant differences between control subjects and remission stage of patients [ （4.73±3.95）×10^-5, P 〉 0.05 ]. The level of butyric acid was significantly higher in the controls [4.53 （2.68,5.47）] ×10^-5 than that in the active stage of patients with HSP [ 1.48 （0.14,3.78） ×10^-5, P 〈 0.017 ] and the remission stage of patients [ 0.93 （0.31,1.75 ） ×10^-5, P 〈 0.017 ]. Compared with the active stage of patients, the level of acetic acid and butyric acid in the remission stage of patients was not significantly different. No significant differences were found in the results of propionic acid, or period.

关 键 词：过敏性紫癜 儿童 粪便 短链脂肪酸 

分 类 号：R72[医药卫生—儿科]