UFLC法同时测定黄芩中4种活性成分的含量  

UFLC Simultaneous Determination of Four Active Components in Scutellaria Radix

作　　者：关皎[1] 朱鹤云[1] 王佳林[1] 姜吉[1] 郝乘仪[1] 郭淑英[1] 冯波[1] 

机构地区：[1]吉林医药学院药学院,吉林吉林132013

出　　处：《中华中医药学刊》2017年第7期1864-1866,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：吉林省卫生厅科研项目(2013Z008);吉林省科技厅科研项目(200905113)

摘　　要：目的:建立超快速液相色谱法同时测定黄芩中黄芩苷、汉黄芩苷、黄芩素和汉黄芩素的含量。方法:采用Shim-Pack XR-ODS柱(75 mm×3.0 mm,2.2μm);流动相为0.1%磷酸水溶液(A)-乙腈(B)梯度洗脱(0~11 min,35%~41%B;11~12 min,41%~35%B),平衡时间2 min;流速为0.4 m L·min-1;检测波长为275nm;柱温为30℃。结果:黄芩苷、汉黄芩苷、黄芩素和汉黄芩素分别在20~200μg·mL^(-1)(r=0.999 6)、4.0~40μg·mL^(-1)(r=0.999 5)、0.1~1.0μg·mL^(-1)(r=0.999 3)和0.4~4.0μg·mL^(-1)(r=0.999 6)浓度范围内与峰面积呈良好的线性关系;平均回收率(n=9)分别为99.2%,98.2%,98.7%和99.1%。结论:该方法结果准确、操作简便、具有良好的重现性和稳定性,可用于黄芩药材的质量控制。Objective： To develop an ultra -fast liquid chromatography （UFLC） method for simuhaneous detelTnina- tions of baicalin, wogonoside, baicalein and wogonin in Scutellaria Radix. Methods ： Chromatographic separation was per- formed on Shim- Pack XR- ODS column（75 mm ×μ 3.0 mm, 2.2 μm）. The mobile phase was 0.1% phosphoric acid （ A ） - aeetonitrile （ B ） with gradient elution （ 0 - 11 rain, 35 % - 41% B ; 11 - 12 min,41% - 35 % B ） at a flow rate of 0.4 mL · min^-1 and the equilibration time was 2 min. The detecting wavelength was set at 275 nm. The column tempera- ture was maintained at 30 ℃. Rusults ： The linear ranges were 20 - 200 μg · mL^-1（ r = 0. 999 6） for baicalin, 4.0 - 40 μg · mL^-1 （ r = 0. 999 5 ） for wogonoside, 0.1 - 1.0 μg· mL^-1 （ r = 0. 999 3 ） for baicalein and 0.4 - 4.0 μg· mL - （ r = 0. 999 6） for wogonin. The average recoveries （ n = 9） of four components were 99.2%, 98.2%, 98.7% and 99. 1%. Rusults： The developed method is simple, accurate with high repeatbility and stability. It is suitable for the quality control of Scutellaria Radix.

关 键 词：黄芩 黄芩苷 汉黄芩苷 黄芩素 汉黄芩素 超快速液相色谱法 

分 类 号：R284.1[医药卫生—中药学]