舒肝宁注射液对大鼠酒精性肝纤维化的防治作用研究  

Study on the Preventive and Therapeutic Effect of Shuganning Injection on Alcoholic Liver Fibrosis in Rats

作　　者：潘婷[1] 张金娟[1] 熊英[1] 李玲[1] 雷珊[1] 兰金芝 

机构地区：[1]贵州医科大学基础医学院,贵阳550025

出　　处：《中国药房》2017年第19期2624-2627,共4页China Pharmacy

基　　金：贵州省科技计划课题(No.黔科合重大专项字[2011]6019号)

摘　　要：目的:考察舒肝宁注射液对酒精性肝纤维化(ALF)模型大鼠的防治作用,为其临床用于酒精性肝病的治疗提供实验参考。方法:取50只大鼠,每天ig 60%乙醇-玉米油-吡唑混悬液复制ALF模型;另取同批大鼠10只,ig生理盐水,作为正常对照组。连续16周后,将存活的造模大鼠(n=40)随机分为模型组、阳性对照组(安络化纤丸0.75 g/kg,ig)和舒肝宁注射液高、中、低剂量组(4.8、2.4、1.2 m L/kg,ip),每组8只。正常对照组和模型组大鼠ip等体积生理盐水(5 m L/kg),给药组大鼠给予相应药物,每天1次,连续给药8周,并同时继续进行造模处理。给药结束后,称定大鼠体质量,检测大鼠血清中肝功能指标[天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)]和肝纤维化指标[透明质酸(HA)、层黏连蛋白(LN)、Ⅲ型前胶原(PⅢNP)和Ⅳ型胶原(Ⅳ-C)]水平,测定大鼠肝指数并观察肝组织病理变化。结果:与正常对照组比较,模型组大鼠体质量显著降低(P<0.05);肝指数以及血清中肝功能指标和肝纤维化指标水平均显著升高(P<0.05或P<0.01);肝组织出现脂肪变性、肝细胞空泡化、汇管区周围有大量纤维组织沉积等病理改变。与模型组比较,各给药组大鼠上述变化均显著改善(P<0.05或P<0.01)。结论:舒肝宁注射液可明显改善ALF模型大鼠的肝组织损伤,对酒精性肝病有一定的防治作用。OBJECTIVE：To investigate the preventive and therapeutic effect of Shuganning injection on alcoholic liver fibrosis（ALF）in model rats,and provide experimental basis for its clinical application for alcoholic liver disease. METHODS：50 rats were enrolled and intraperitoneally given mixed liquid of 60% alcohol-corn oil-pyrazole to reduce ALF model. Another 10 rats were enrolled and intraperitoneally given normal saline,as normal control group. After 16 weeks,survived model rats（n=40）were randomly divided into model group,positive control group（Anluo huaxian pill 0.75 g/kg,ig）,Shuganning injection high-dose,medium-dose,low-dose groups（4.8,2.4,1.2 m L/kg,ip）,8 in each group. Normal control group and model group were intraperitoneally injected equal volume of normal saline（5 m L/kg）,administration groups were given relevant medicines,once a day,for 8weeks;and modeling was contiuously conducted at the same time. After administration,body mass of rats was weighed,and the levels of liver function indexes [aspartate aminotransferase（AST）,alanine aminotransferase（ALT）] and liver fibrosis indexes [hyaluronic acid（HA）,laminin（LN）,type Ⅲ procollagen（PⅢNP）,type Ⅳ collagen（Ⅳ-C）] in serum of rats were detected. Liver index of rats was determined and pathological changes of liver tissue were observed. RESULTS：Compared with normal control group,body mass of rats in model group was significantly decreased（P〈0.05）;liver index,and liver function index,liver fibrosis index levels in serum were significantly increased（P〈0.05 or P〈0.01）. Liver tissue showed steatosis,hepatocyte vacuolization,a large number of fibrous tissue deposition around portal areas and other pathological changes. Compared with model group,above-mentioned changes were improved significantly in administration groups（P〈0.05 or P〈0.01）. CONCLUSIONS：Shuganning injection can obviously improve liver tissue damage of model rats with ALF,showing certain preventive and therapeutic effect on alco

关 键 词：舒肝宁注射液 酒精性肝纤维化 大鼠 

分 类 号：R285[医药卫生—中药学]