正交试验优化益骨颗粒的提取工艺  

Optimization of Extraction Technology of Yigu Granule by Orthogonal Test

作　　者：彭红英 江涛 杨滨宾 陈雪华 陈晓坚 刘敏珊 

机构地区：[1]广州白云山敬修堂药业股份有限公司研究所,广州510130

出　　处：《中国药房》2017年第19期2684-2687,共4页China Pharmacy

基　　金：广州市科技计划项目(No.2012J4300070)

摘　　要：目的:优化益骨颗粒的提取工艺。方法:采用L_9(3~4)正交试验,以淫羊藿苷、龙胆苦苷、马钱苷酸转移率和出膏率的综合评分为评价指标,以乙醇体积分数、溶剂用量、提取次数、提取时间为考察因素,优化益骨颗粒的提取工艺并进行工艺验证。结果:优化的提取工艺为药材加10倍量60%乙醇回流提取2次,每次1 h。验证试验结果表明,淫羊藿苷、龙胆苦苷、马钱苷酸的平均转移率分别为81.28%、48.71%、59.82%(RSD分别为1.54%、2.37%、2.52%,n=3),平均出膏率为31.48%(RSD=1.97%,n=3)。结论:优化的益骨颗粒提取工艺稳定可行、重复性好,可为后续的生产工艺研究提供依据。OBJECTIVE：To optimize the extraction technology of Yigu granule. METHODS：L_9（3~4）orthogonal test was used,using comprehensive scores of transfer rate of icariin,gentiopicroside,loganic acid and yield rate as evaluation indexes,alcohol volume fraction,the amount of solvent,extraction times and extraction time as investigation factors,extraction technology of Yigu granule was optimized,and the verification test was conducted. RESULTS：The optimized extraction technology was 10-fold 60%ethanol reflux extraction for twice,1 h once. Results of verification test showed,average transfer rates of icariin,gentiopicroside and loganic acid were 81.28%（RSD=1.54%,n=3）,48.71%（RSD=2.37%,n=3）and 59.82%（RSD=2.52%,n=3）;average yield rate was 31.48%（RSD=1.97%,n=3）. CONCLUSIONS：The optimized extraction technology for Yigu granule is stable and feasible with good reproducibility,which can provide basis for the follow-up study of production process.

关 键 词：益骨颗粒 正交试验 提取工艺 淫羊藿苷 龙胆苦苷 马钱苷酸 

分 类 号：R284.2[医药卫生—中药学]