基于数学模拟研究曼氏无针乌贼(Sepiella japonica)在不同温度下进食后胃内容物特性变化及胃排空规律  

EFFECTS ON CHANGES OF STOMACH CONTENTS AND GASTRIC EVACUATION OF SEPIELLA JAPONICA AFTER EATING AT DIFFERENT TEMPERATURES BASED ON MATHEMATICAL SIMULATION

作　　者：平洪领 余方平[1] 张涛[1] 史会来[1] 

机构地区：[1]浙江省海洋水产研究所浙江省海水增养殖重点实验室,舟山316021

出　　处：《海洋与湖沼》2017年第3期647-655,共9页Oceanologia Et Limnologia Sinica

基　　金：2015浙江省科技计划项目;2015F50055号;2015年浙江省海洋与渔业局项目(乌贼和大黄鱼放流亲体种质复壮及扩繁);2016年浙江省科技计划项目;2016F50041号;国家级星火计划项目;2015GA700014

摘　　要：为研究曼氏无针乌贼(Sepiella japonica)在不同温度下(15oC、20oC和25oC)进食冰鲜葛氏长臂虾(Palaemon gravieri)后的胃内容物特性变化及胃排空规律,本实验将采用3种常用数学模型(线性、平方根及指数模型)对胃排空曲线进行拟合,比较进食后胃排空过程中胃内容物的水分、pH和消化酶的变化规律。结果表明,3种数学模型均能较好地描述曼氏无针乌贼胃排空曲线,但是平方根模型对曼氏无针乌贼胃内容物湿重和干重的数据拟合效果最优。根据平方根模型计算,在15oC、20oC和25oC水温时曼氏无针乌贼50%胃内容干重排出的理论时间分别为4.4h、3.7h和3.6h,而完全排空的时间分别为13.8h、12h和12h。在摄食后0—14h内,胃内容物水分含量表现为先逐渐下降后显著上升;pH值在摄食后0—8h内逐渐降低,8—14h缓慢上升;胃蛋白酶和胰蛋白酶均呈现先升高后降低的变化趋势,而淀粉酶则总体保持恒定。曼氏无针乌贼在15oC、20oC和25oC三个温度下进食冰鲜葛氏长臂虾,胃排空过程中的胃内容物水分含量、pH值和消化酶比活力均存在显著差异(P<0.05)。综上所述,25oC水温条件更有利于曼氏无针乌贼的胃排空过程,同时建议在饲喂管理中按照3.6h的间隔进行投喂。To study the changes of stomach contents and gastric evacuation of Sepiella japonica after feeding Palaemon gravieri at different temperatures（15？C, 20？C and 25？C）, three common mathematical models（linear, square root and exponential model） were used to fit the gastric evacuation curve, while water content, pH and digestive enzymes in stomach contents were analyzed during gastric evacuation process. The results showed that three kinds of mathematical models all were able to describe the gastric evacuation curve, however, the square root model had the best effect on the data of wet weight and dry weight within stomach contents of S. japonica. The theoretical time that excreting 50% dry weight of stomach contents was 4.4h, 3.7h and 3.6h respectively at 15？C, 20？C and 25？C, with completely emptying was 13.8h, 12 h and 12 h. During 0—14h after feeding, the water of the stomach contents decreased gradually before drastically decreasing; the pH value decreased slowly at initial 8h and increased at followed 6h. Both pepsin and trypsin showed the trend that increase followed by decrease, but amylase remained generally stable. The water content, pH value and digestive enzyme specific activity of stomach contents in the process of gastric evacuation were significant（P0.05） at the temperature of 15？C, 20？C and 25？C. To summarize, the 25？C water temperature conditions are more favorable for the gastric evacuation of S. japonica, and it is recommended to feed at the interval of 3.6h in feeding management.

关 键 词：曼氏无针乌贼 温度 胃排空 数学模拟 胃内容物 

分 类 号：Q482[生物学—生理学] S968[农业科学—水产养殖]