Effect of plum-blossom needle tapping with different stimulation intensities on hair regrowth in hair removal mice  

皮肤针不同刺激量对脱毛小鼠毛发生长的影响（英文）

作　　者：刘志丹 汪青良 付蓓 李晓燕 

机构地区：[1]Department of Acupuncture, Shanghai Baoshan Hospital of Integrated Traditional Chinese Medicine and Western Medicine [2]Department of Integrative Medicine, Shanghai Dermatology Hospital [3]School of Nursing, Hubei University of Traditional Chinese Medicine

出　　处：《Journal of Acupuncture and Tuina Science》2017年第3期184-190,共7页针灸推拿医学（英文版）

基　　金：supported by Shanghai Baoshan Hospital of Integrated Traditional Chinese and Western MedicineNo.201405;Excellent TCM Doctor Training Project in the Three-year Traditional Chinese Medicine Action Plan from Shanghai Health and Family Planning Commission,No.ZY3-RCPY-2-2041;Youth Medical Talents Training Project from Baoshan District Health and Family Planning Commission of Shanghai,BSWSYQ-2014-A05~~

摘　　要：Objective： To determine the role of intensity of plum-blossom needle tapping in treating alopecia areata. Methods： The BALB/c mice were randomized into a normal group, a control group, a roller-needle（RN） group, a mild plum-blossom needle（MP） group, and a heavy plum-blossom needle（HP） group. An area of hair was removed by external application of 8% sodium sulfide on BALB/c mice. The hair regrowth, hair follicle changes, and local inflammatory factor changes after cutaneous acupuncture were observed. Results： After treated with sodium sulfide, the hair was completely removed, the local hair follicles reached the catagen phase, and the expressions of interleukin（IL）-1α, IL-1β, IL-2, tumor necrosis factor（TNF）-α, interferon（IFN）-γ, and IL-17 were increased. Mice intervened by RN achieved the same hair growth rating as the controls but with thicker hair shafts; mice in the MP group had incomplete and uneven hair growth but thicker hair shafts; mice in the HP group didn＇t show hair growth. Pathological analysis revealed significant inflammatory infiltration into the local follicle bulbs and increased catagen-phase follicles in the control group, while RN and MP groups showed significantly increased anagen-phase follicles, coarser individual hairs, and obvious hair shafts. Meanwhile, most of the hair follicles in the HP group were in telogen phase and showed obvious surrounding inflammatory infiltration. RN, MP, and HP significantly down-regulated the increased IL-2, IL-1α, IL-1β, TNF-α, and IFN-γ levels（P〈0.05）, but didn＇t notably affect the increased CD34 expression（P〈0.05）. Conclusion： Cutaneous acupuncture with heavy stimulation intensity can inhibit hair growth in hair removal mice, while RN, with the lightest stimulation intensity, is unlikely to affect hair growth but may make hair shafts thicker and follicles larger.目的:观察不同皮肤针刺激量对脱毛小鼠毛发生长的影响。方法:BALB/c小鼠随机分为正常组、对照组、滚针组、梅花针轻叩组、梅花针重叩组。8%硫化钠外涂使小鼠局部脱毛。皮肤针治疗后观察毛发生长、毛囊改变及局部炎症因子变化。结果:小鼠局部经8%的硫化钠涂抹后毛发完全脱落,毛囊进入退行期,炎症细胞因子白介素(IL)-1α、IL-1β、IL-2、肿瘤坏死因子(TNF)-α、干扰素(IFN)-γ、IL-17表达增加。滚针组小鼠毛发生长完全程度与模型对照组相同,但毛干较模型对照组更粗大;梅花针轻叩组小鼠毛发生长不完全、欠均匀,但毛干较模型对照组粗大;梅花针重叩组小鼠毛发未生长。病理显示对照组脱毛小鼠局部毛囊球部炎症浸润明显,进入退行期的毛囊增多;滚针组、梅花针轻叩组与对照组比较,处于生长期的毛囊明显增多、个体粗大,并可见明显毛干;梅花针重叩组毛囊多进入静止期,且周围炎性浸润明显。免疫组化显示滚针、梅花针轻叩、梅花针重叩治疗均能使增高的IL-2、IL-1α、IL-1β、TNF-α、IFN-γ下降(P<0.05),但对增高的CD34表达无明显作用(P>0.05)。结论:刺激量大的梅花针重叩抑制了毛发的生长;刺激量最轻的滚针,不影响毛发生长,而且能使毛干、毛囊更粗大。

关 键 词：ALOPECIA Cutaneous Acupuncture Plum-blossom Needle Therapy Roller Needle INTERLEUKIN Inflammation MICE 

分 类 号：R2-03[医药卫生—中医学]