UHPLC-QTOF-MS法分析鉴定吴茱萸水提物在大鼠血浆、尿液和粪便中的原形成分及其代谢产物  

Analysis of major bioactive components and their metabolites in rats' plasma, urine and feces after oral administration of the aqueous extract of Euodiae Fructus by UHPLC-QTOF-MS

作　　者：程宇欣 陈两绵[2] 王智民[2] 张启伟[2] 闫利华[2] 赵正保[1] 

机构地区：[1]山西医科大学,山西太原030001 [2]中国中医科学院中药研究所中药质量控制技术国家工程实验室/国家中医药管理局中药过程控制技术重点研究室,北京100700

出　　处：《药学学报》2017年第7期1157-1164,共8页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(31200244);中医药行业科研专项资助项目(201507002-3)

摘　　要：采用UHPLC-QTOF-MS技术,对吴茱萸水提物的主要成分及给药后大鼠体内的原形成分及其代谢产物进行定性分析。通过分析各成分的保留时间、精确分子质量、一级、二级质谱信息并与相应的对照品比对,从吴茱萸水提物中鉴定了27个成分,其中25个成分经对照品确证;从灌胃给予吴茱萸水提物的大鼠血浆、尿液和粪便中鉴定、推测出16个原形成分和35个代谢产物,16个原形成分均经对照品确证。吴茱萸水提物的主要成分有酚酸、黄酮苷、柠檬苦素和生物碱。生物碱、黄酮和酚酸存在于血浆、尿液和粪便中,柠檬苦素主要存在于尿液和粪便中。这些成分在大鼠体内代谢的主要途径有羟基化、氢化、甲基化、硫酸化、葡糖醛酸化等。该研究基本明确了吴茱萸水提物中各类成分在大鼠体内的主要存在形式,为吴茱萸体内药效物质的阐明提供了参考。An ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry（UHPLC-Q-TOF/MS） method has been established to analyze the bioactive components of the aqueous extract of Euodiae Fructus and detect the metabolites in rats after oral administration of the aqueous extract. Compounds were detected by analyzing their retention times, high resolution mass data, mass spectra and comparing with the reference substances. As a result, 27 compounds were characterized from the aqueous extract of Euodiae Fructus, and 25 compounds were identified on the basis of the reference substances. 16 parent compounds and 35 metabolites were detected in rats＇ plasma, urine and feces samples, and all of the parent compounds were determined unambiguously through comparison with the reference substances. Four classes of compounds were identified from the aqueous extract of Euodiae Fructus, including phenolic acids, limonins, flavonoid glycosides and alkaloids. Phenolic acids, flavonoid glycosides and alkaloids were detected in rats＇ plasma, urine and feces, while limonins were detected in the urine and feces. The main metabolism pathways of these compounds in rats might include hydroxyl, hydrogenation, methylation, sulfating, glucuronidation, and so on. The results of this study provide references in the material basis of Euodiae Fructus in vivo.

关 键 词：吴茱萸 水提物 原形成分 代谢产物 超高效液相色谱-飞行时间质谱联用 

分 类 号：R917[医药卫生—药物分析学]