七宝美髯口服液对小鼠脑组织SOD活力及羰基化蛋白含量的影响  

Effects of Qibao Meiran Oral Liquid on SOD activity and carbonyl protein content in brain tissue of mice

作　　者：孟诗[1] 魏江平[1] 郑航[1] 吴倩[1] 付昆[1] 张占军[2] 徐世军[1] 

机构地区：[1]成都中医药大学四川省中药资源系统研究与开发利用国家重点实验室培育基地,四川成都611137 [2]北京师范大学认知神经科学与学习国家重点实验室,北京100875

出　　处：《中成药》2017年第7期1347-1350,共4页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金重点项目(81430100);四川省学术和技术带头人培养资金资助项目(2015058);中药药理四川省青年科技创新研究团队项目(2014TD0007)

摘　　要：目的观察七宝美髯口服液(制何首乌、当归、补骨脂等)对SAMP8小鼠学习记忆功能、海马组织病理形态及SOD活力、羰基化蛋白含有量的影响。方法 27只SAMP8小鼠随机均分为模型对照组、盐酸多奈哌齐组及七宝美髯口服液组,另取9只SAMR1小鼠作为正常对照组,连续灌胃60 d,在给药第56天进行避暗训练,5 d后测定学习记忆能力;HE染色观察病理形态;ELISA法检测脑组织中SOD活力和羰基化蛋白含有量。结果与模型对照组比较,七宝美髯口服液组小鼠的逃避潜伏期显著延长,错误次数明显降低(P<0.01),海马组织的病理形态显著改善,SOD活力显著升高,羰基化蛋白含有量明显降低(P<0.01)。结论七宝美髯口服液能改善SAMP8小鼠的学习记忆功能,减轻海马组织的退行性病变程度。AIM To observe the effects of Qibao Meiran Oral Liquid （Polygoni multifiori Radix Praeparata, Angelicae sinensis Radix, Psoraleae Fructus, etc. ） on learning and memory function, hippocampus tissue patholog-ical morphology, SOD activity and carbonyl protein content in SAMP8 mice. METHODS Twenty-seven SAMP8 mice were randomly and equally divided into model control group, donepezil hydrochloride group and Qibao Meiran Oral Liquid group. Another nine SAMR1 mice were selected as normal control group. Mice were given successive intragastric administration for 60 days. On the 56th day, the passive avoidance test was adopted, and the learning and memory capacities were determined after 5 d; The pathological morphology was observed by HE staining; ELISA assay was used to detect the activity of SOD and the content of carhonyl protein in brain tissue. RESULTS Compared with the model control group, the escape latency of mice in the Qibao Meiran Oral Liquid group was significantly prolonged, and the number of errors decreased significantly （ P 〈 0.01 ） ; the pathological morphology of hippocampus tissue was significantly improved ; SOD activity increased significantly, and carbonyl protein content decreased significantly （P 〈 0. 01 ）. CONCLUSION Qibao Meiran Oral Liquid can not only improve the learn- ing and memory function of SAMP8 mice, but also reduce the degree of hippocampus tissue degenerative disease.

关 键 词：七宝美髯口服液 退行性病变 SOD 羰基化蛋白 SAMP8小鼠 

分 类 号：R285.5[医药卫生—中药学]