木豆叶HPLC指纹图谱建立及2种成分测定  

Establishment of HPLC fingerprints of Cajanus cajan leaves and determination of two constituents

作　　者：胥爱丽[1,2] 毕晓黎 李素梅 李养学 江洁怡 刘布鸣[3] 

机构地区：[1]广州中医药大学,广东广州510006 [2]广东省中医药工程技术研究院(广东省中医药研究开发重点实验室),广东广州510095 [3]广西中医药研究院(广西中药质量标准研究重点实验室),广西南宁530022

出　　处：《中成药》2017年第7期1435-1439,共5页Chinese Traditional Patent Medicine

基　　金：广东省科技计划项目(2013B040200040;2015B070701010);佛山市科技创新专项资金(2013GQ100032);广西中药质量标准研究重点实验室开放课题资助项目(桂中重开201402)

摘　　要：目的建立木豆Cajanus cajan(L.)Millsp.叶HPLC指纹图谱,并测定荭草苷和木犀草素的含有量。方法木豆叶65%甲醇提取液的分析采用Agilent Zorbax SB-C18色谱柱(4.6 mm×250 mm,5μm);以甲醇-1%醋酸为流动相,梯度洗脱;体积流量1.0 m L/min;柱温25℃;检测波长260 nm。结果 10批样品(S1~S10)HPLC指纹图谱中有21个共有峰,除S3(0.516)外,其相似度均大于0.950。荭草苷和木犀草素分别在0.089 5~3.960μg和0.015 5~0.408μg范围内呈良好的线性关系,平均加样回收率分别为99.43%(RSD=1.32%)和98.50%(RSD=0.82%),3个产地(广东、云南、海南)样品中2种成分含有量有明显差异。结论该方法简单准确,重复性好,可用于木豆叶的质量控制。AIM To establish the HPLC fingerprints of Cajanus cajan （L.） Millsp. leaves and to determine the contents of orientoside and luteolin. METHODS The analysis of 65% methanol extract from C. cajan leaves was performed on a 25 ℃ thermostatic Agilent Zorbax SB-Cls column （4. 6 mm ×250 mm, 5 μm）, with the mo- bile phase comprising of methanol-1% acetic acid flowing at 1.0 mL/min in a gradient elution manner, and the de- tection wavelength was set at 260 nm. RESULTS There were twenty-one common peaks in ten batches of samples （S1-S10）, whose similarities were more than 0. 950, except for that of S3 （0. 516）. Orientoside and luteolin showed good linear relationships within the ranges of 0.089 5 -3. 960 μg and 0. 015 5 -0. 408 μg, whose average recoveries were 99.43% （ RSD = 1.32% ） and 98.50% （ RSD = 0. 82% ）, respectively. The contents of two con- stituents in the samples from three growing areas （Guangdong, Yunnan and Hainan） showed obvious differences. CONCLUSION This simple, accurate and reproducible method can be used for the quality control of C. cajan leaves.

关 键 词：木豆 叶 HPLC指纹图谱 荭草苷 木犀草素 

分 类 号：R284.1[医药卫生—中药学]