太子参多糖的提取纯化及其单糖组分的鉴定  

Extraction,Purification and Monosaccharide Composition Analysis of Polysaccharides from Radix Pseudostellariae

作　　者：王慧娟[1,2] 乔杨[1] 晏薇娜 董庆洁[1] 杨坤仙 周英[1,2] WANG Huijuan QIAO Yang YAN Weina DONG Qingjie YANG Kunxian ZHOU Ying(School of Pharmaceutical Science, Guizhou University, Guiyang 550025, China Guizhou Medicine Edible Plant Resource Application Development Engineering Laboratory, Guiyang 550025, China)

机构地区：[1]贵州大学药学院,贵州贵阳550025 [2]贵州省药食两用资源应用开发工程实验室,贵州贵阳550025

出　　处：《贵州大学学报（自然科学版）》2017年第3期25-29,共5页Journal of Guizhou University:Natural Sciences

基　　金：施秉中药材产业科技合作专项计划(施中药科合专项2013);贵州省药食同源资源研究开发科技创新人才团队(黔科合人才团队[2015]4010号);贵州省药食两用资源应用开发工程实验室(黔发改投资[2015]542号)

摘　　要：目的:通过提取纯化得到太子参精制多糖,并对其单糖组成进行鉴定分析。方法:水提醇沉后的多糖采用Sevag法除去蛋白,再经Sephadex G-100凝胶柱层析进一步纯化,透析冻干后制得纯度较高的精制多糖。高温强酸条件下将多糖进行水解,加入1-苯基-3-甲基-5-吡唑啉酮(PMP)溶液衍生化后采用HPLC法分析单糖组成。结果:精制太子参多糖的含量为82.30%,主要由葡萄糖、半乳糖、木糖、鼠李糖等单糖组成。结论:本研究可为太子参药材质量标准的提升完善提供基础。Objective： To obtain high purity polysaccharides from radix pseudostellariae by extraction and purification process,then analyze the monosaccharide composition of the polysaccharides. Methods： The crude polysaccharide was prepared by water-extraction and alcohol-precipitation method,then proteins was eliminated by Sevag method. Crude sample was further purified by using Sephadex G-100 gel column chromatography. High purity polysaccharides powder was finally obtained by casting the dialysed solution into freeze dryer. The polysaccharide was hydrolyzed to monosaccharide in high-temperature and acidic condition,then the hydrolysate was derivatized with 1-phenyl-3-methyl-5-pyrazolone（ PMP） and analyzed by HPLC. Results： The content of high purity polysaccharides powder is 82.30%. It mainly comprises glucose,galactose,xylose and rhamnose. Conclusion： This research can provide basic reference information for the improvement of follow-up quality standard of radix pseudostellariae.

关 键 词：太子参多糖 单糖组分 柱前衍生化 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]