拜赖青霉活性产物的抗真菌作用研究  

Study on the Antifungal Effect of Bioactive Metabolites Produced by Penicillium bilaiae

作　　者：张楠楠[1,2] 白净[2] 张礼文[2] 徐玉泉[2] 王惠国[1] 冯宝民[1] ZHANG Nannan BAI Jing ZHANG Liwen XU Yuquan WANG Huiguo FENG Baomin(School of Life Science and Biotechnology, Dalian University, Liaoning Dalian 116622, China Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China)

机构地区：[1]大连大学生命科学与技术学院,辽宁大连116622 [2]中国农业科学院生物技术研究所,北京100081

出　　处：《生物技术进展》2017年第4期315-319,共5页Current Biotechnology

基　　金：国家973计划项目(2015CB755700);农业部948项目(2016-X43)资助

摘　　要：采用平板对峙实验和生长速率法,筛选和测定拜赖青霉ACCC 30440对尖孢镰刀菌与大丽轮枝菌的抑制活性。结果表明,ACCC 30440的PDB发酵液和乙酸乙酯提取物均能够抑制尖孢镰刀菌与大丽轮枝菌的生长,含10%发酵液的培养基对尖孢镰刀菌与大丽轮枝菌的抑制率均达40%以上,含20%发酵液则可完全抑制这两株病原真菌的生长;ACCC30440的乙酸乙酯提取物对尖孢镰刀菌与大丽轮枝菌的半抑制浓度分别为518.63μg/m L和443.42μg/m L。说明拜赖青霉ACCC 30440菌株能够产生抑制植物病原真菌生长的活性物质。The antagonistic activity of PeniciUium bilaiae ACCC 30440 against Fusarium oxysporum and VerticiUium dahliae was screened and determined by plate confrontation experiment and growth rate method. The results showed that ACCC 30440 fermentation broth and ethyl acetate extract could inhibit the growth of F. oxysporum and V. dahliae, and the inhibition rate to F. oxysporum and V. dahliae were both more than 40% with 10% fermentation broth, while 20% fermentation broth completely inhibited the growth of these two pathogenic fungi. The half inhibitory concentrations of ACCC 30440 ethyl acetate extract on F. oxysporum and V. dahliae were 518.63 μg/mL and 443.42 μg/mL, respectively. The results indicated that P. bilaiae ACCC 30440 strain could produce bioactive metabolites that inhibited the growth of phytopathogenic fungi.

关 键 词：拜赖青霉 尖孢镰刀菌 大丽轮枝菌 活性产物 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]