C-反应蛋白损伤内皮细胞的蛋白质组学研究  

Proteomics Analysis of C-relative Protein Effect on Endothelial Cell

作　　者：刘少军[1] 胡坤华[2] 刘慰华[1] LIU Shao-jun HU Kun-hua LIU Wei-hua(Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, China)

机构地区：[1]广州医科大学附属第二医院,广东广州510260 [2]中山大学中山医学院

出　　处：《中国医学创新》2017年第19期15-18,共4页Medical Innovation of China

基　　金：广东省省级科技计划项目(2014A020212327);广州市属高校科技计划项目(1201410344)

摘　　要：目的:从蛋白质组学的角度探讨C-反应蛋白(CRP)损伤内皮细胞(EC)的作用机制。方法:采用培养的人冠状动脉内皮细胞HCAEC第4代用于实验,25μg/mL CRP刺激HCAEC 24 h,2D DIGE结合MALDI-ToF-ToF质谱鉴定进行蛋白质组学分析研究。结果:发现差异表达蛋白斑点11个,鉴定出其中4个,包括Heat shock cognate 71 kDa protein、Elongation factor 1-beta和Proteasome activator complex subunit 1等。结论:通过蛋白质组学研究,筛选了CRP损伤EC的差异表达蛋白,为深入理解EC损伤的分子机制提供有益的线索。Objective： To investigate the role of CRP in endothelial cell by proteomics analysis. Method： Human coronary artery endothelial cells were used at passage 4.25 μ g/mL CRP served as stimulus for endothelial cell injury.Differentially expressed proteins were analyzed by 2D DIGE and identified by MALDI- ToF-ToF MS analysis.Result： Differentially expressed 11 spots were visualized and 4 were identified, including Heat shock cognate 71 kDa protein, Elongation factor 1-beta and Proteasome activator complex subunit 1.Conclusion： This study screen differentially expressed proteins by CRP stimulus via proteomics analysis, providing useful understand of the underlying molecular mechanism of endothelial cell injury.

关 键 词：C-反应蛋白 内皮细胞 蛋白质组学研究 动脉粥样硬化 

分 类 号：R541.4[医药卫生—心血管疾病]