宁夏贺兰山东麓酿酒葡萄病毒病田间自然发病率调查及检测  

Natural Incidence Investigation and RT-PCR Detection of Wine Grapes Virus Disease in Helan Mountain East Region of Ningxia

作　　者：吕苗苗[1] 纳莹 孙牧笛 徐全智[1] 顾沛雯[1] 

机构地区：[1]宁夏大学农学院,宁夏银川750021

出　　处：《北方园艺》2017年第13期55-62,共8页Northern Horticulture

基　　金：宁夏教育厅资助项目(NGY14011);宁夏"十三五"科技重大专项资助项目(NX2016)

摘　　要：对宁夏贺兰山东麓8个酿酒葡萄种植地区8个葡萄品种的病毒病田间自然发病情况进行调查,采用RT-PCR方法,检测了40份样品中GLRaV-1~5这5种葡萄卷叶伴随病毒的感染率。结果表明:宁夏贺兰山东麓酿酒葡萄主要病毒病有葡萄卷叶病、扇叶病和栓皮病,其中葡萄卷叶病尤为严重;不同酿酒葡萄种植区和品种间葡萄卷叶病的感染存在着差异,老葡萄种植区中品种间葡萄卷叶病发病率明显高于新葡萄种植区的品种;"蛇龙珠"和"黑比诺"是感染葡萄卷叶病毒的主要品种,发病率高达85.1%和52.7%;利用5种葡萄卷叶伴随病毒引物进行RT-PCR检测,检出3种病毒类型,且GLRaV-3的检出率最高,为87.5%。Eight wine grape planting areas in Helan Mountain East Region of Ningxia for grape virus diseases natural infection situation were investigated, by adopting RT-PCR method to test 40 samples of five kinds of grape leaf roll virus infection from GLRaV-1 to 5. The results showed that the major wine grape virus diseases in Helan Mountain East Region of Ningxia include grapevine leaf roll disease, grapevine fan leaf disease and grapevine corky bark, meanwhile grapevine leaf roll disease was particularly serious. There was difference between different wine grape planting areas as well as wine grape varieties of grape leaf roll disease infection. And the varieties of leaf roll disease incidence in the old grape wine region were obviously higher than the new wine region; ＇Cabernet Gernischt＇ and ＇Pinot Gris~ were the main grape varieties with grape leaf roll-associated virus with incidence rate as high as 85.1% and 52.7% By RT-PCR detection for five kinds of grape leaf roll-associated virus primers,three types of viruses were diagnosed with GLRaV-3 acting out the highest detection rate at 87. 5%.

关 键 词：宁夏贺兰山东麓 酿酒葡萄病毒病 田间自然发病率 葡萄卷叶伴随病毒(GLRaVs) RT-PCR检测 

分 类 号：S436.631.19[农业科学—农业昆虫与害虫防治]