狗牙根内生固氮菌对结缕草的促生效应  

Promoting Effect of Endophytic Diazotrophs From Bermudagrass on Zoysia japonica Steud

作　　者：武鑫[1,2] 李凤娇[1,2] 刘天增[1,2] 张巨明[1,2] 

机构地区：[1]华南农业大学林学与风景园林学院,广东广州510642 [2]华南农业大学草业工程技术研究中心,广东广州510642

出　　处：《北方园艺》2017年第13期113-118,共6页Northern Horticulture

基　　金：广东省科技计划资助项目(2012B020302002);广东省自然科学基金资助项目(2015A030310200)

摘　　要：以结缕草为试材,将分离自野生狗牙根的内生固氮菌DQ-3活化测定活性后制成2种浓度菌液(1×10~3、1×10~6 cfu·mL^(-1)),再将长势相同的结缕草茎段于菌液中浸泡4、16h后进行盆栽试验,采用测定成坪后植株的颜色、分蘖数、地上地下生物量及植株全氮量的方法,研究内生固氮菌对结缕草的促生效果,以探索不同草种间内生固氮菌的通用性。结果表明:菌液浓度为1×10~6 cfu·mL^(-1),浸泡4h对结缕草分蘖数促进最显著;菌液浓度为1×10~6 cfu·mL^(-1),浸泡16h对颜色的影响最大;菌液浓度为1×10~6 cfu·mL^(-1),浸泡16h对地上生物量影响最大;菌液浓度为1×10~3 cfu·mL^(-1),浸泡4h对地下生物量影响最大;菌液浓度为1×10~6 cfu·mL^(-1),浸泡16h对结缕草植株增加全氮量效果最好。综合看来,菌液浓度为1×10~6 cfu·mL^(-1),浸泡16h的组合对结缕草促生效果最好。Taking Zoysia japonica Steud as test materials,the endophyte diazotroph QD-3 was isolated from bermudagrass and made into two different concentration bacteria liquid（1 ×103 cfu · mL-1 and 1×106 cfu·mL-1）. After soaking Zoysia japonica Steud stems with similar growth for 4 hours and 16 hours respectively, the potting test began. When turf established, the turf color, tillers number, aboveground biomass,underground biomass and total nitrogen were evaluated to study the promoting effect of endophytic diazotrophs on zoysia grass. In the meanwhile, the versatility between different grass species could also be explored. The results showed that soaking under the concentration of 1 × 106 cfu· mL- 1 for 4 hours had the most promoted tillers number;soaking under the concentration of 1×106 cfu · mL- 1 for 16 hours had well-influenced turf color; soaking under the concentration of 1 × 106 cfu ·mL-1 for 16 hours showed the most improved aboveground biomass while soaking under the concentration of 1× 103 cfu · mL-1 for 4 hours had greater underground biomass. Soaking under the concentration of 1 × 106 cfu · mL , for 16 hours had the best promotion in total nitrogen of Zoysia japonica Steud. In conclusion,soaking under the concentration of 1 × 106 cfu · mL-1 for 16 hours had the best promoting effect on Zoysia japonica Steud.

关 键 词：内生固氮菌 接种 结缕草 促生作用 

分 类 号：S543.9[农业科学—作物学]