白念珠菌分泌的天冬氨酸蛋白酶6诱导人单核细胞凋亡  

Apoptosis of human monocytes induced by secretory aspartic proteinase 6 secreted by Candida albicans

作　　者：崔凡[1] 李丹[1] 陈霞[1] 王有为[1] 杨戈[1] CUI Fan LI Dan CHEN Xia WANG You-wei YANG Ge(Institute of Dermatology,Sichuan A- cademy of Medical Sciences ＆ Sichuan Provincial People＇s Hospital,Chengdu 610031 ,China)

机构地区：[1]四川省医学科学院.四川省人民医院皮肤病性病研究所,四川成都610031

出　　处：《实用医院临床杂志》2017年第4期30-32,共3页Practical Journal of Clinical Medicine

基　　金：国家自然科学基金青年基金资助项目(编号:81101231);四川省卫生和计划生育委员会科研基金资助项目(编号:150195)

摘　　要：目的检测白念珠菌分泌的天冬氨酸蛋白酶6(Sap6)对人类单核细胞系(THP-1细胞)增殖的影响,明确Sap6蛋白是否具有诱导单核细胞凋亡的活性。方法用XTT比色法检测25、50μg/ml Sap6对THP-1细胞增殖率的影响;在流式细胞仪下用Annexin V-FITC/PI细胞凋亡试剂盒检测25、50μg/ml Sap6诱导THP-1细胞凋亡情况;用Con A-FITC/DAPI双染法在荧光显微镜下观察Sap6作用于THP-1细胞的位置。结果与25μg/ml比较,50μg/ml Sap6蛋白显著抑制人THP-1细胞增殖率(P=0.0001),并提高THP-1细胞早期凋亡率(P=0.0001);Sap6蛋白进入THP-1细胞内,结合于细胞核。结论白念珠菌分泌的Sap6在体外能够进入人单核细胞内,诱导凋亡,从而抑制细胞增殖率。Objective To investigate the effects of secretory aspartic proteinase 6 （Sap6） from Candida albicans on human monocyte cell line （ THP-1 ） proliferation, and to find out whether Sap6 has an activity to induce the apoptosis of monocytes. Methods The effect of Sap6 on THP-1 cell proliferation was evaluated by XTF assay. Apoptotic rate of THP-1 cells induced by Sap6 was meas- ured by flow cytometry with Annexin V-FITC/PI apoptosis detection kit. The location of Sap6 on THP-1 ceils was examined by ConA- FITC/DAP1 staining under fluorescence microscope. Results 50 μg/ml of Sap6 had a significantly inhibitory effect on the proliferative rate of THP-1 （ P = 0. 0001 ）. 50 μg/ml of Sap6 increased the early apoptotic rate of THP-1 （ P = O. 0001 ）. Sap6 was internalized into THP-1 and combined with nucleus of the cells. Conclusion Secretory aspartic proteinase 6 from Candida albicans could access into human monocytes in vitro, and inhibit the proliferation of monocytes by inducing cell apoptosis.

关 键 词：白念珠菌 人单核细胞系 分泌型天冬氨酸蛋白酶6 细胞凋亡 

分 类 号：R379.4[医药卫生—病原生物学]