纯钛表面镁离子改性后磷灰石沉积、蛋白质吸附及成骨细胞初期反应的研究（英文）  

In vitro Apatite Formation,Protein Adsorption and Initial Osteoblast Responses on Titanium Surface Enriched with Magnesium

作　　者：徐玲利[1] 史兴岭[1] 欧阳春[1] 刘文[1] 

机构地区：[1]江苏科技大学,江苏镇江212003

出　　处：《稀有金属材料与工程》2017年第6期1512-1517,共6页Rare Metal Materials and Engineering

基　　金：Priority Academic Program Development of Jiangsu Higher Education Institutions;Doctor Project of Jiangsu University of Science and Technology(635061402)

摘　　要：通过一种简单可行的方法对钛表面进行镁离子改性。首先将纯钛放入高浓度氢氧化钠(NaOH)溶液中进行碱化处理,然后再转移至稀氯化镁(MgCl_2)溶液中进行离子交换,最后进行热处理,在纯钛试样表面形成了含有钛酸镁成分的3D纳米网状结构。对试样的表面形貌、粗糙度和化学组成进行了分析;考察了试样在模拟人工体液中诱导羟基磷灰石沉积的能力;以牛血清白蛋白为模型,研究了表面改性对纯钛试样蛋白吸附能力的影响;评价了MC3T3-E1成骨细胞在试样表面的附着、铺展和增殖。结果表明,与钠(Na)离子改性相比,镁离子改性加快了羟基磷灰石在试样表面的沉积,并明显促进了蛋白吸附。此外,镁离子改性促进了成骨细胞附着、铺展及随后的增殖。A feasible treatment to modify titanium with Mg was carried out.Briefly,pure titanium was treated in condensed sodium hydroxide solution first and then transferred to diluted magnesium chloride solution to conduct ion exchange.After that,heat treatment was conducted and a nano-sized network containing magnesium titanate was obtained.Surface morphology,roughness,and chemical composition were characterized.In vitro apatite inducing ability was evaluated in simulated body fluid（SBF） and bovine serum albumin（BSA） was used as model to study protein adsorption.MC3T3-E1 cells were cultured and initial cell attachment,morphology,proliferation were evaluated.Results show that compared with sodium（Na） modified surface,Mg immobilization accelerates apatite formation and promotes protein adsorption significantly.Besides,cell attachment is improved and cell spreading is enhanced on Mg-containing samples compared with Na containing samples.Increased early cellular attachment results in subsequent increase of number of proliferated cells on the Mg-containing surface.

关 键 词：钛 骨传导性 镁 磷灰石 蛋白质 成骨细胞 

分 类 号：R318.08[医药卫生—生物医学工程] TG174.4[医药卫生—基础医学]