阴道蝇蛆病对阿拉善双峰驼外周血清中细胞因子IL-4、IL-10、IFN-γ含量的影响  

Influence of vaginal magoots inoculation on IL-4,IL-10,IFN-γ content in peripheral serum in Alxa bactrian camels

作　　者：姜建强[1] 刘图雅 戴钦 安希文 图雅 包花尔[1] 王瑞[1] 王秀珍[1] 李盈 额尔敦木图[1] JIANG Jian-qiang LIU Tu-ya DAI Qin AN Xi-wen TU Ya BAO Hua-er WANG Rui WANG Xiu-zhen LI Yings Eerdunmutu(College ture Key Laboratory of Animal Disease Clinical of Veterinary Medicine/The Ministry of Agricul- Diagnosis and Treatment Technology, Inner Mongolia Agricultural University, Hohhot 010018, China Veterinary Bureau of Alxa Left Banner, Ba yanhot, Inner Mongolia 750300,China Husbandry Service Center of Alxa Economic Development Zone ,Alxa, Inner Mongolia 750336, China Detachment of Alxa Agriculture and Animal Husbandry Comprehensive Administrative Law Enforcement, Bayanhot, Inner Mongolia 750300, China Veterinary Bureau of Erguna , Hulunbeir , Inner Mongolia 022250 ,China)

机构地区：[1]内蒙古农业大学兽医学院/农业部动物疾病临床诊疗技术重点实验室,内蒙古呼和浩特010018 [2]内蒙古阿拉善左旗农牧业局兽医局,内蒙古巴彦浩特750300 [3]阿拉善经济开发区农牧业服务中心,内蒙古阿拉善750336 [4]内蒙古阿拉善盟农牧业综合行政执法支队,内蒙古巴彦浩特750300 [5]内蒙古呼伦贝尔额尔古纳市兽医局,内蒙古呼伦贝尔022250

出　　处：《中国兽医学报》2017年第7期1343-1347,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31360591);内蒙古自治区自然科学基金资助项目(2012MS0421);留学回国人员科研启动基金资助项目(教外司留[2011]1139号)

摘　　要：试验选取6峰12岁健康双峰母驼,试验组与对照组各3峰。周期14d,分为一期(感染前,1~3d)、二期(发病期间,4~12d)、三期(蝇蛆落地后,12~14d),每天采集血液获得血清保存,于第4天对试验组进行人工感染蝇蛆。结果显示:对照组一期与试验组一期3种细胞因子含量均无显著性差异(P>0.05);对照组二期与试验组二期IL-10、IFN-γ含量差异显著(P<0.05),试验组含量较高,IL-4含量差异不显著(P>0.05);在三期中,试验组IL-4含量较低,IFN-γ含量较高且与对照组比较均差异显著(P<0.05)。对照组一、二、三期之间3种细胞因子含量差异均不显著(P>0.05);试验组IL-4含量一、二、三期间无显著性差异(P>0.05),IL-10二期含量高于一期且差异显著(P<0.05),IFN-γ二、三期含量均高于一期且差异显著(P<0.05)。结果表明:阴道蝇蛆病对双峰驼外周血清中IL-4含量基本没影响,但会使IL-10、IFN-γ含量升高,在蝇蛆落地后IL-10含量会降低,IFN-γ仍维持在较高水平。This experiment intended to explorewhether there is a relationship between interleukin- 4（IL-4） ,interleukin-10（IL-10）,interferon-γ（IFN-γ） content in peripheral serum in Alxa bactrian Ii camels and the vaginal myiasis. Six 12-year-old healthy female camels were chosen,three were as experiment group and anothers were as control group. The experimental period lasted 14 days and it was divided into three parts including the first period-FP（before the treat）,the second period-SP （during the treat）, the third period-TP（after maggots landing）, serum samples were every day. At the fourth day, the experiment camels were infected with maggots. Compared with control group FP and the experiment group FP, there was no significant difference among the three eytokines （P〉0.05） ;except the IL-4, the IL-10 and IFN-γ contents in the experiment group SP were significantly higher than the control group SP （P〈0.05）. In the TP,the experiment group had lower IL- 4 content and higher IFN-γ content（P〈0.05） compared with control group. In the three periods of the control group,there was on significant difference in the three cytokines. Both the IL-10 content and the IFN-γ content in the experiment group FP were significantly higher than those of the control group FP （P 〈 0. 05）. In the experiment group IL-4 content did not have a significant change in the three periods（P〉0.05）. Vaginal maggots infection has no impact on the IL-14 content in peripheral serum in Alxa bactrian camels,but it would rise the IL-10 and IFN-γ content, but the IL-10 content would be reduced and the IFN-γ content remained at a high level after maggots landing.

关 键 词：阿拉善双峰驼 白细胞介素-4 白细胞介素-10 干扰素-Γ 蝇蛆 

分 类 号：S855.9[农业科学—临床兽医学]