高效液相色谱法测定清脑降压片中橙黄决明素和大黄酚的含量  

Determination of Aurantio Obtusin and Chrysophanol in Qingnaojiangya Pian by HPLC

作　　者：张桂英[1] 郭宝凤 

机构地区：[1]乌兰察布医学高等专科学校基础医学部,乌兰察布012000 [2]内蒙古自治区食品药品检验所,呼和浩特010000

出　　处：《中国医药导刊》2017年第5期531-532,共2页Chinese Journal of Medicinal Guide

摘　　要：目的:建立清脑降压片中橙黄决明素和大黄酚的含量测定方法。方法:采用高效液相色谱法,色谱柱为C_(18)柱(250mm×4.6mm,5μm);流动相为乙腈-0.1%磷酸水梯度洗脱;橙黄决明素、大黄酚检测波长为284nm;428nm;流速为1.0ml·min^(-1);柱温35℃。结果:橙黄决明素在14.98~149.98ng范围内与峰面积积分值呈良好的线性关系y=0.0001,X-0.4539(γ=1.0000)。平均加样回收率为98.7%,RSD为1.86%(n=6)。大黄酚在4.00~40.00ng范围内与峰面积积分值呈良好的线性关系(γ=1.0000),Y=0.0004,X-0.046。平均加样回收率为99.0%,RSD为2.02%(n=6)。结论:本方法准确、精密、重复性好,可作为清脑降压片中橙黄决明素和大黄酚的含量测定方法。Objective：To establish a Qingnaojiangya Pian of aurantio obtusin and chrysophanol content determination method.Methods：The chromatographic saparation was carried out at 35℃ column temperature on a Phenomen C18 column （250mm x 4.6mm,5μm） using acetonitrile-0.1% phosphoric acid water gradient elution;The flow rate was 1.0 mL · min-1;Aurantio obtusin and chrysophanol detection wavelength of 284nm;the detection wavelength was 428nm.Results：Aurantio obtusin element in the 14.98 ～149.98ng the scope of internal and peak area integral value showed a good linear relationship Y =0.0001X-0.4539 （r =1.0000）.The average recovery rate was 98.7% and RSD was 1.86% （n =6）.Chrysophanic acid in the 4.00 ～40.00ng range and the peak area integral value is good linear relationship Y =0.0004X-0.046 （r =1.0000）.The average recovery rate was 99% and RSD was 2.02% （n =6）.Conclusion：The method is accurate,precise,reproducible good,as in Qingnaojiangya tablets of aurantio element and chrysophanol content determination method.

关 键 词：清脑降压片 高效液相色谱法 橙黄决明素 大黄酚 

分 类 号：O657.72[理学—分析化学] R286.0[理学—化学]