全反式维甲酸对人胃腺癌细胞株SGC-7901凋亡的影响  

Effect of all-retinoic acid on the apoptosis of human gastric cancer SGC-7901 cells

作　　者：魏翔[1] 陈绍龙[1] 贾宁[1] 周青[1] 李静静[2] 张素梅[1] 汪渊[1] 

机构地区：[1]安徽医科大学分子生物学实验室生物化学与分子生物学教研室安徽省省部共建教育部重要遗传病基因资源利用重点实验室,合肥230032 [2]安徽公安职业学院,合肥230031

出　　处：《安徽医科大学学报》2017年第8期1119-1122,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81201907);高校省级优秀青年人才基金项目(编号:2012SQRL067);安徽医科大学博士科研基金(编号:XJ201229)

摘　　要：目的研究全反式维甲酸(ATRA)对胃腺癌细胞系SGC-7901凋亡的影响,并初步探讨其可能的分子机制。方法 ATRA处理SGC-7901细胞,Hoechst染色检测ATRA对SGC-7901细胞凋亡的影响,Western blot法检测ATRA对凋亡相关蛋白表达情况的影响。结果 ATRA可诱导SGC-7901细胞的凋亡,且下调凋亡抑制蛋白Bcl-2的表达,对促凋亡蛋白Bax的表达和酶原形式的Caspase-3的表达没有影响。结论 ATRA促进SGC-7901细胞的凋亡,其分子机制可能是下调Bcl-2的表达,使Bcl-2/Bax的值降低进而促进细胞凋亡。Objective To study the effect of all retinoic acid （ ATRA） on the apoptosis of human gastric cancer lines SGC-7901 cells,and to investigate the possible mechanism of apoptosis. Methods SGC-7901 gastric cancer cells were treated by ATRA. Hoechst staining was used to detect the effect of ATRA on the apoptosis of SGC-7901 cells,and the effect of ATPR on the expression of apoptosis related protein was detected by Western blot. Results All trans retinoic acid （ ATRA） could induce the apoptosis of SGC-7901 cells and downregulate the expression of Bcl-2, but had no effect on the expression of pro-apoptotic protein Bax and Caspase-3. Conclusion ATRA pro-motes the apoptosis of SGC-7901 cells in gastric cancer cells. The molecular mechanism may be related to reduce the expression of Bcl-2, so that decrease the value of Bcl-2 /Bax and then promote cell apoptosis.

关 键 词：全反式维甲酸 胃癌 SGC-7901 凋亡 

分 类 号：R453.3[医药卫生—治疗学] R977.3[医药卫生—临床医学]