TSG-6在大鼠静脉桥血管增生中的表达及意义  

The expression and significance of TSG-6 in rat vein graft hyperplasia

作　　者：张彪[1] 张成鑫[1] 龚文辉[1] 许金国[1] 葛圣林[1] 

机构地区：[1]安徽医科大学第一附属医院心脏外科,合肥230022

出　　处：《安徽医科大学学报》2017年第8期1160-1163,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省科技厅重点科研资助项目(编号:1301043025)

摘　　要：目的观察肿瘤坏死因子α刺激基因-6(TSG-6)在大鼠移植静脉壁中的表达,探讨其作用及可能的机制,为临床治疗移植后血管再狭窄提供新方向和理论依据。方法采用袖套法将大鼠自体颈外静脉移植入同侧颈动脉制作血管移植模型,实验分为假手术组、支架组和非支架组。术后12周切除移植静脉,应用苏木精-伊红观察移植静脉内膜、中膜、外膜增生情况,采用计算机图像分析系统测量和计算各膜厚度;应用Western blot检测移植静脉壁内TSG-6、肿瘤坏死因子(TNF)-α和白介素(IL)-1的表达。结果假手术组和支架组的TSG-6、TNF-α和IL-1表达水平、移植静脉内膜增生程度均显著低于非支架组(P<0.05)。结论 TSG-6在大鼠静脉桥血管中表达明显升高,并与TNF-α、IL-1炎症因子表达呈正相关性;外支架可能通过抑制移植静脉的炎症反应而限制移植血管壁增生。Objective To observe the expression of tumor necrosis factor alpha-stimulated gene-6 （TSG-6） in the rat transplanted vein graft, and to explore its action and possible mechanism to provide a new direction and theoreti-cal basis for the clinical treatment of vascular restenosis after vein transplantation. Methods T he vascular graft model was made by cuff technique, in which the autograft of the external jugular vein of the rat was transplanted in-to the ipsilateral carotid artery. The experiment was divided into three groups s h a m operation group, stent group and non-stent group. Then the grafts were removed 12 weeks postoperatively. Hematoxylin-eosin staining was used to observe the proliferation of mesenteric vein, medial and adventitial vein. The thickness of the grafts was meas-ured and analyzed by immunohistochemistry with computer image analysis system. Western blot was used to detect TSG- 6, TNF-a and IL -1. Results The expression of TSG-6, TNF-a and IL -1 in the sham operation group and the stent group were significantly lower than those in the non-stent group （P 〈0. 05） . Conclusion T he expression of TSG-6 in vein grafts of rats is significantly increased and correlated with the expression of TNF-a and IL -1. Th e ex-ternal stent may inhibit the proliferation of transplanted vascular wall by inhibiting the inflammatory reaction of graft vein.

关 键 词：TSG-6 桥血管 血管外支架 内膜增生 

分 类 号：R654[医药卫生—外科学] R541.4[医药卫生—临床医学]