榅桲果实及种子不同提取部位的蛋白酪氨酸磷酸酶1B抑制活性筛选  

Screening the protein tyrosine phosphatase 1B inhibitory activities of the extracts from various components of Cydonia oblongafruits and seeds

作　　者：阿米乃木.买买提 布造热.艾合买提 赵海清[3] 哈及尼沙[1] 

机构地区：[1]新疆医科大学药学院,乌鲁木齐830011 [2]新疆建设职业技术学院计算机信息工程学院,乌鲁木齐830054 [3]中国科学院新疆理化技术研究所,乌鲁木齐830011

出　　处：《西北药学杂志》2017年第4期400-402,共3页Northwest Pharmaceutical Journal

基　　金：国家自然科学基金项目(编号:31360078)

摘　　要：目的探讨榅桲果实和种子的甲醇提取物及其不同溶剂提取部位对蛋白酪氨酸磷酸酶1B(PTP1B)的抑制作用,筛选抗糖尿病活性部位。方法利用PTP1B体外筛选系统,对榅桲果实和种子不同提取部位进行细致筛选,确定其降糖活性部位。结果榅桲果实和种子各部位均有不同程度抑制PTP1B活性的作用,其中榅桲种子乙酸乙酯部位、榅桲果实二氯甲烷部位和榅桲果实水部位的体积分数为50%的乙醇溶液洗脱部位对PTP1B抑制作用强,其半数抑制质量浓度(IC50)分别为0.844,0.423和0.395mg·L-1,初步推测榅桲果实及种子提取物具有抗2型糖尿病的潜力。结论榅桲种子乙酸乙酯部位、榅桲果实二氯甲烷部位和榅桲果实水部位的体积分数为50%的乙醇溶液洗脱部位是主要的抗糖尿病活性部位。Objective To screen the protein tyrosine phosphatase 1B（PTP1B）inhibitory activities of the methanol extracts from Cydonia oblongafruits and seeds different parts,and to find out the anti-diabetic site.Methods Various components fromCydonia oblongawere screened using PTP1 Bin vitro system to identify their hypoglycemic active fraction.Results The various extracted part of Cydonia oblongafruits and seeds showed different inhibitory effects on the PTP1 Bactivity,among which,the ethyl acetate extract from seeds,CH2Cl2 extract from fruits and 50% ethanol extract from fruits showed the strong inhibition effect on the PTP1 Bwith IC50values of 0.844,0.423 and 0.395mg·L^-1,respectively.Conclusion The ethyl acetate extract part from seeds,CH2Cl2 extract part from fruits and 50% ethanol extract part from fruits of Cydonia oblonga were main anti-diabetic active parts.

关 键 词：榅桲 蛋白酪氨酸磷酸酶1B 抑制作用 

分 类 号：R285.5[医药卫生—中药学]