丁香酚对大鼠肾缺血再灌注性损伤保护作用的研究  

Protective effects of eugenol on renal ischemia-reperfusion injury in rats

作　　者：刘启祥 谢家恩 张立冬 吴明勇 

机构地区：[1]重庆市开州区人民医院泌尿外科,重庆405400 [2]重庆市开州区人民医院肿瘤科,重庆405400

出　　处：《临床和实验医学杂志》2017年第13期1261-1264,共4页Journal of Clinical and Experimental Medicine

摘　　要：目的探讨丁香酚(EUG)对大鼠肾缺血再灌注(RI/R)损伤的保护作用及相关分子机制。方法肾动脉夹闭法制作肾缺血30 min再灌注12 h模型,90只SD大鼠随机分为3组:对照组,模型组和实验组,每组30只。其中实验组制作动物模型前采用丁香酚200 mg/kg预处理2周,对照组,模型组给予等体积生理盐水。化学定量法检测大鼠血清肌酐(Scr)、尿素氮(BUN),酶联免疫吸附(ELISA)法检测血清和肾组织丙二醛(MDA)、超氧化物歧化酶(SOD),并利用Western blot和免疫组织化学染色染色检测肾脏组织NF-κB蛋白表达。结果化学定量结果显示,模型组大鼠血清Scr、BUN较对照组显著提高(P<0.05),而实验组丁香酚可显著降低大鼠血清Scr、BUN,与模型组比较差异有统计学意义(P<0.05);Elisa结果显示,与对照组相比,模型组大鼠血清和肾组织MDA含量显著增高,SOD水平显著降低(P<0.05),而丁香酚可显著降低大鼠血清和肾组织MDA含量,提高SOD水平(P<0.05);Western blot及免疫组织化学染色结果显示,丁香酚可明显抑制蛋白NF-κB蛋白表达(P<0.05)。结论丁香酚可通过降低氧化应激水平和抗NF-κB抑制大鼠肾缺血再灌注性损伤。Objective To explore the effects of eugenol on renal ischemia-reperfusion injury in rats and its potential mechanism.Methods Renal ischemia-reperfusion injury model was made via arterial clipping method.90 rats were divided into 3 groups： the control group,model group and test group.Rats in test group were pre-treated with 200 mg/kg eugenol for 2 weeks,rats in the control group and model group were treated with equal saline.Serum creatinine （Scr） and urea nitrogen （BUN） were detected by chemical quantification.Malondialdehyde （MDA） and superoxide dismutase （SOD） in serum and kidney tissue were detected by elisa.The protein expression of NF-κB were valued by western blot and immunohistochemical staining.Results Chemical quantification showed that Scr and BUN in model group were significantly higher than those in the control group （P＆lt;0.05）,and eugenol could markedly suppress the Scr and BUN level （P＆lt;0.05）.Elisa showed that MDA in serum and kidney tissue in model group were significantly higher than that of the control group （P＆lt;0.05）,and the activity of SOD were significantly lower than that of the control group （P＆lt;0.05）,while,eugenol could markedly suppress the content of MDA （P＆lt;0.05））,and up-regulated the activity of SOD in serum and kidney tissue （P＆lt;0.05）.Western blot and immunohistochemical staining showed that eugenol could markedly suppress the protein expression of NF-κB in kidney tissue （P＆lt;0.05）.Conclusion Eugenol can protect the renal ischemia-reperfusion injury via its anti-oxidation and anti-apoptosis pathway.

关 键 词：大鼠 肾 缺血再灌注 丁香酚 抗氧化 

分 类 号：R285.5[医药卫生—中药学]