Bcl-2基因转染骨髓干细胞治疗激素性股骨头坏死的疗效研究  

Treatment of steroid-induced femoral head necrosis with Bcl-2 gene modified bone marrow mesenchymal stem cells

作　　者：张立岩[1] 孙新[2] 田丹[2] 罗勇[3] 陈继营[4] 柴伟[4] 王华[1] 徐睿[1] 刘鑫[1] 王海涛[1] 

机构地区：[1]北华大学附属医院骨外1科,吉林吉林132001 [2]北华大学生命科学研究中心,吉林吉林132001 [3]中国矿业大学材料学院,江苏徐州221116 [4]解放军总医院,北京100853

出　　处：《中国现代医学杂志》2017年第14期19-23,共5页China Journal of Modern Medicine

基　　金：吉林省科技发展计划(No:20150312013ZG);吉林省教育厅"十二五"科学技术研究项目[No:吉教科合字(2015)第141号];吉林省教育厅"十三五"科学技术研究项目[No:吉教科合字(2016)第63号]

摘　　要：目的研究Bcl-2基因转染的骨髓间充质干细胞(BMSCs)结合髓芯减压法对兔早期激素性股骨头坏死的疗效。方法使用共计40只家兔复制早期激素性股骨头坏死模型,其中成功的模型家兔共28只。随机分为4组:空白对照组6只,不作任何处理;髓芯减压组6只,单纯减压;BMSCs治疗组8只,在减压同时植入BMSCs;Bcl-2治疗组8只,在减压同时植入Bcl-2基因转染的BMSCs。植入后分别在第8、12周各取股骨头标本行病理学检查及骨细胞凋亡检测,观察骨细胞生长及凋亡情况。结果成功复制兔早期激素性股骨头坏死动物模型;培养出BMSCs后进行传代;Bcl-2基因转染成功;治疗12周后,Bcl-2治疗组动物的一般情况改善,组织切片和细胞凋亡检测结果显示,Bcl-2治疗组的空骨陷窝和骨细胞凋亡数量与其他3组比较,差异有统计学意义(P<0.05)。结论使用髓芯减压将Bcl-2基因修饰的BMSCs移植到兔早期激素性股骨头坏死动物模型体内,具有较好的成骨治疗作用。Objective To explore the curative effect of implantation of bone marrow mesenchymal stem cells （BMSCs） transfected with Bcl-2 combined with core decompression on experimental femoral head necrosis in rabbits. Methods Forty rabbits were selected, and femoral head necrosis models were successfully prepared in 28 rabbits. Then, the model rabbits were randomized into 4 groups： blank control group （n =6） without treat-ment, core decompression group （n=6）, BMSCs group （n = 8） subjected to core decompression and implantation of BMSCs, and Bcl -2 group （n =8） subjected to core decompression and implantation of Bcl -2-transfected BMSCs. In the 8th and 12th week after implantation, femoral head tissues were taken and sliced into sections for pathological examination and detection of osteocyte apoptosis. Results BcJgene was successfully trans-fected into BMSCs. At the 8th week after implantation, there was only a little new bone formation in the core decompression group and the BMSCs group; at the 12th week, fibrous tissues were formed in the decompression channel. In the Bcl -2 group, new bone formation was observed, and at the 12th weeks, the necrotic region was completely repaired. The positive TUNEL staining of the Bcl-2 group was much less than that of the other groups, the differences were significant（P〈 0.05）. Conclusions These findings indicate that Bcl -2-transfected bone marrow mesenchymal stem cell transplantation via core decompression has better curative effect on exper-imental femoral head necrosis in rabbit model.

关 键 词：骨髓间充质干细胞 BCL-2基因 股骨头坏死 髓芯减压 

分 类 号：R684[医药卫生—骨科学]