实时荧光定量PCR法在淋病奈瑟菌感染检测中的应用效果分析  

Real time fluorescent quantitative PCR method in the analysis of application effect in the detection of Neisseria gonorrhoeae infection

作　　者：韦敏[1] 覃李线[1] 粟永俊[1] 

机构地区：[1]广西省南宁市第一人民医院检验科,广西南宁530022

出　　处：《吉林医学》2017年第7期1258-1261,共4页Jilin Medical Journal

摘　　要：目的:探讨实时荧光定量PCR法在淋病奈瑟菌感染检测中的应用效果。方法:选取淋病奈瑟菌感染检测患者298例为研究对象,分别采用培养检测法及实时荧光定量PCR法从检测线性范围、检测重复性、检测灵敏度、检测特异性及检测试剂稳定性等方面进行比较。结果:经两组方法比较,在检测线性范围、检测重复性、检测灵敏度、检测特异性及检测试剂稳定性等方面,PCR检测法显示了一定的优势,但也存在一定的漏检率。结论:临床上应充分应用各种检测方法的优势,使之相互补充,以提升淋病奈瑟菌的检出率。Objective To evaluate the efficacy of real - time quantitative PCR in detection of Neisseria gonorrhoeae infection. Methods 298 cases of Neisseria gonorrhoeae infection were selected as the subjects. The methods of culture detection and real -time fluorescence quantitative PCR were used to compare linear range, repeatability, sensitivity, specificity and stability of de-tection reagent respectively. Results The two groups of comparison, in the linear range of detection, detection repeatability, de-tection sensitivity, specificity and stability of detection reagent, PCR detection method showed some advantages, but there were also some missing rate. Conclusion The advantages of various detection methods should be fully applied to complement each oth-er in order to improve the detection rate of Neisseria gonorrhoeae.

关 键 词：实时荧光定量PCR 淋病奈瑟菌感染 培养检测法 应用效果 

分 类 号：R446.1[医药卫生—诊断学] R759.2[医药卫生—临床医学]