‘红阳’猕猴桃全基因组AP2/EREBP转录因子生物信息学分析  

Genome-wide bioinformatics analysis of AP2/EREBP transcription factor in 'Hongyang' kiwifruit

作　　者：邓浪[1,2] 沈兵琪 王连春[1,2] 周军[1,2] 刘惠民[1,2] 周凡[1,2] 李德龙[1,2] 

机构地区：[1]西南林业大学云南省高校林木遗传育种改良与繁育重点实验室,昆明650224 [2]西南林业大学西南山地森林资源保育与利用省部共建教育部重点实验室,昆明650224

出　　处：《果树学报》2017年第7期790-805,共16页Journal of Fruit Science

基　　金：国家林业局948项目"猕猴桃新品种及分子育种研究技术引进"(2012-4-62);云南省林学一流学科建设经费;云南省高校林木遗传改良与繁育重点实验室开放基金;西南林业大学大学生创新基金(C16063)

摘　　要：【目的】分析‘红阳’猕猴桃全基因组AP2/EREBP转录因子家族。【方法】利用Kiwifruit Genome Database、EMBI、TAIR、SMART、Pfam、MEME、Protparam、SOPM、SWISS-MODEL、Signal P4.1 Sever网站,和Clustal X2、Bio Edit、MEGA6.0、Map Inspect、MEV软件,分析‘红阳’猕猴桃AP2/EREBP转录因子类型、结构域、系谱进化、蛋白理化性质及高级结构、信号肽分析、基因定位、序列元件和基因表达模式。【结果】‘红阳’猕猴桃全基因组中有204条AP2/EREBP转录因子,根据其结构域划分为4个亚家族。进行多序列比对和系谱进化分析,发现2个亚家族各分为6个亚组。生物信息学分析表明,204条蛋白氨基酸序列高级结构与拟南芥AP2/EREBP转录因子相似性较高。其中存在2条分泌型蛋白。基因定位表明,该家族基因在10号染色体无定位;有染色存在串联复制现象。同源拟南芥基因表达模式分析表明,AP2/EREBP转录因子对外界胁迫有显著性表达。【结论】利用生物信息学方法获得204条猕猴桃AP2/EREBP家族转录因子,并与拟南芥AP2/EREBP转录因子进行系谱进化、结构域、基因定位和同源表达等分析,结果表明猕猴桃AP2/EREBP转录因子在进化过程中比较保守,并参与了植物发育和胁迫应答调控。[Objective] AP2/EREBP transcription factor family is a plant-specific transcription factor, which plays an important role in plant growth, development and stress response. It contains at least one AP2 binding domain and about 60-70 highly conserved amino acids. The function of the AP2/EREBP transcription factor family of the genome of Actinidia chinensis was analyzed with the completion of se- quencing of the full genome of ＇Hongyang＇ kiwifruit. [Methods ] AP2/EREBP family transcription factors were analyzed by bioinformatics. The protein and nucleic acid sequence of AP2/EREBP transcription fac- tor of kiwifruit and Arabidopsis were obtained by Kiwifruit Genome Database and TAIR database. And the domains of the AP2/EREBP transcription were analyzed by SMART, Pfam. The AP2/EREBP transcription factor was used for sequence element analysis. The domain sequence mapping was analyzed by MEME. In order to analyze the evolutionary relationship of 204 AP2/EREBP transcription factors in ＇ Hon-gyang＇ kiwifruit, ClustalX2 and MEGA6.0 were used to sequence the AP2/EREBP chinensis and Arabidopsis thaliana, and to carry out the phylogenetic analysis. The erties, secondary structure, tertiary structure, and signal peptide analysis of the AP transcripts of Actinidia Physicochemical prop- 2/EREBP transcription factor protein sequence of Actinidia chinensis were carried out using Protparam, SOPM, SWISS-MODEL and SignalP4.1 Sever. The Kiwifruit AP2/EtlEBP transcription factor sequence information was obtained from the Kiwifruit Genome Database. The MapInspect tool was used to construct the gene mapping map. The Arabidopsis thaliana AP2/EREBP transcription factor local database was constructed using BioEdit software. Blast was used to obtain the Arabidopsis thaliana sequence of AP2/EREBP transcription factor, and the Arabidopsis thaliana expression data was downloaded from EBI. The MEV tool was used to log in the downloaded data and to make an expression heat-map. [Results] The protein and nucleic acid se- quence of AP2/EREBP

关 键 词：猕猴桃 AP2/EREBP 转录因子 生物信息学分析 

分 类 号：S663.4[农业科学—果树学]