巴西蕉2个ERF转录因子基因的克隆及功能研究  

Cloning and characterization of two ERF transcription factor genes in Brazil banana

作　　者：侯晓婉 胡伟[2] 徐碧玉[2] 张鲁斌[1] 金志强[2] 

机构地区：[1]中国热带农业科学院南亚热带作物研究所.海南省热带园艺产品采后生理与保鲜重点实验室,广东湛江524091 [2]中国热带农业科学院热带生物技术研究所.农业部热带作物生物学与遗传资源利用重点实验室,海口571101

出　　处：《果树学报》2017年第7期806-816,共11页Journal of Fruit Science

基　　金：现代农业产业技术体系(国家香蕉产业技术体系)建设专项(CARS-32);973计划前期研究专项(2014CB160314);香蕉;杧果;菠萝保鲜;贮运关键技术集成与产业化应用(ZDZX2013011)

摘　　要：【目的】筛选、克隆响应非生物胁迫的关键ERF转录因子基因,并研究其功能。【方法】综合运用转录组学、生物信息学、生物化学等技术手段,分析不同非生物逆境胁迫下AP2/ERF转录因子超家族基因的表达谱聚类变化,并从中筛选、克隆关键ERF转录因子,对其功能进行研究。【结果】不同非生物胁迫下,AP2/ERF超家族基因的表达模式不同,对低温胁迫的响应较其他胁迫敏感;筛选克隆的Ma ERF25和Ma ERF27基因是ERF家族基因,具备ERF家族基本特征,编码蛋白为核定位蛋白,C端具有转录激活活力,并参与了香蕉对非生物胁迫和激素的应答,在其中发挥着一定的作用。【结论】获得了2个巴西蕉ERF基因Ma ERF25和Ma ERF27,被定位在细胞核,具有转录激活活性,参与非生物逆境胁迫应答。[ Objective ] Banana （Musaceae, Musa） is one of the most important food plants, widely ing in tropical and subtropical countries. Banana is particularly sensitive to most of the abiotic distribut- stresses, such as freezing, drought and salt, which would cause heave decrease of the yield and quality. Therefore, investigation the molecular mechanism of the response of banana to abiotic stresses is of prime importance for improving stress-resistant ability. Moreover, over the last decades APETALA2/Ethylene Responsive Factor （AP2/ERF） proteins have been found to be involved in a variety of biological processes. Based on the number of the structure domain, AP2/ERF superfamily was divided into four families as AP2/ERF, ERF, RAV and soloist. The proteins of ERF family only contained an AP2/ERF structure domain and were studied more deeply than others in all transcription factors family. The genes of ERF family played an important role in regulating plant response to abiotic stresses. The purpose of this study is to clone the key ERF transcription factors of banana and to identify their functions in response to abiotic stresses in or- der to provide a basis for improving stress-resistant ability. [ Methods ] The relative transcriptome results of AP2/ERF superfamily genes of Brazilian banana involved in responding to abiotic stresses of leaves were obtained. The expression profile analysis on AP2/ERF super family genes of banana were conducted by using MeV software. In view of the sensitivity to low temperature stress, MaERF25 and MaERF27 were iso- lated from banana using RNA reverse transcription cDNA mixture from various tissues and organs of ba- nana as template. By using ExPASy, DNAMAN and some other related biological analysis softwares and on the basis of protein sequence homology, the sequences homologue and the basic characteristics of protein were analyzed. In addition, the transient expression vectors containing MaERF25 and MaERF 27 gene were constructed on the basis of plant expression vector pCAMB

关 键 词：香蕉 AP2/ERF 基因克隆 亚细胞定位 转录激活 实时荧光定量PCR 

分 类 号：S668.1[农业科学—果树学]