气管切开插管留置大鼠肺部β-防御素-2及相关免疫指标的改变  

The variation of the respiratory system beta - defensin - 2 and related immune indexs in rats model with tracheotomy intubation by one - time epidural catheter

作　　者：朱翠香[1] 潘宇政[1] 彭玲玲[1] 罗艳[2] 

机构地区：[1]广西医科大学第一附属医院中医科,南宁530021 [2]广西医科大学药学院,南宁530021

出　　处：《中华实验外科杂志》2017年第7期1166-1169,共4页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金（81460687）;广西壮族自治区卫生厅中医药科技专项（GZPT13-36）

摘　　要：目的探讨气管切开插管留置大鼠不同时间段β-防御素-2（rBD2）及相关免疫指标的变化及其规律。方法将无特定病原体（SPF）级健康雄性Sprague—Dawley（SD）大鼠分成正常对照组（A）、气管切开插管留置模型组（B）各24只。制备大鼠气管切开插管留置模型。在造模成功后第24、72、168小时3个时段处死大鼠，观察体重及肺组织形态学改变。采集肺部及外周咀标本，检测rBD2 mRNA的表达、白细胞介素-2（IL-2）及T细胞亚群（CD3^＋、CD4^＋、CD8^＋）的含量。结果（1）B组大鼠肺组织rBD2 mRNA在第24小时表达为2．230±0．521，与A组（0．767±0．269）比较明显升高，而后随着时间的延长其表达（0．229±0．098）逐渐下降，并低于A组（0．909±0．342）水平，至第168小时达到本时间段内最低值（0．107±0．054）。（2）B组外周血CD3^＋含量在第24小时为（10．190±0．876）％，与A组含量（14．390±1．393）％比较明显下降，在第72小时含量（14．040±1．331）％，又恢复至A组大鼠（14．090±1．440）％水平，此后再没有改变。（3）血清IL-2及外周血CD4^＋、CD8^＋的含量，两组比较在3个时间段没有差异，同一组内在3个不同时间段之间的比较也没有差异。（4）B组大鼠体重随着时间的延长逐渐下降，并在第168小时下降最明显[（198．500±8．440）g]。（5）肺组织出现不同程度病变。结论气管切开插管留置大鼠免疫功能变化以肺部局部功能下降为主，这种变化可能与气管切开后肺部损伤和营养不良有关。Objective To investigate the variation in different time period and its regulation of the respiratory system beta- defensin -2 （rBD2） and related immune indexs in rats model with tracheotomy intubation by one -time epidural catheter. Methods Sprague -Dawley （SD） rats were randomly divided into 2 groups with 24 in each group： sham - operated group, model group. Observeing the changes of the weight and lung tissues in rats After modeling. And then, peripheral blood and lung tissues were collected with in 24h, 72 h and 168h respectively, lung tissues rBD2 mRNA expression were measured by reverse transcription - polymerase chain reaction （ RT - PCR）, senun Interleukin - 2 （ IL - 2） content were measured by ELISA, and the peripheral blood T lymphocyte subsets were measured by Flow cytometry respectively. Results I,ung tissues rBD2 mRNA expression （2. 230 ± 0. 521 ） in 24 h increased significantly after modeling, and then began to decline, in the 72 h （0. 229 ± 0. 098） was lower than that in group A （0. 909 ±0. 342）, in the 168 h to A minimum （0. 107 ±0. 054）. Peripheral blood CD3^＋ content （10. 190 ±0. 876）% in 24 h decreased significantly after modeling, back to group A level （14. 040 ± 1. 331 ）% in the 72 h, since there is no change. But Serum IL -2 content and peripheral blood CD4^＋ , CD8^＋ content in group B compared with group A did not differ between three periods, and there was no difference between three differcnt time periods within the same grnup. Group B rats weight （ 198.5 ±8.44） g gradually decline, the lungs appcar different degree of pathological changes. Conclusion The changes of immune function in rats model with tracheotomy intubation by one - time epidural catheter were mainly localized in lung function, which may be related to lung injury and malnutrition after gas cutting.

关 键 词：SPRAGUE-DAWLEY大鼠 气管切开插管留置 Β-防御素-2 白细胞介素-2 T细 胞亚群 

分 类 号：R459.7[医药卫生—急诊医学]